Monocyte chemotactic and activating factor is a potent histamine-releasing factor for human basophils.
Open Access
- 1 February 1992
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 175 (2), 489-493
- https://doi.org/10.1084/jem.175.2.489
Abstract
Recombinant monocyte chemotactic-activating factor (MCAF) has been shown to induce histamine release from human basophils with a dose response between 10(-9) and 10(-6) M. The peak of activity was reached at 10(-7) M. Histamine release by MCAF was rapid with an initial rate comparable with histamine release by an optimal dose of anti-IgE. MCAF led to peak histamine release within 1 min. 80% of the subjects tested were responsive to MCAF or anti-IgE, while all were responsive to FMLP. The percentage histamine release by MCAF was, however, less than that seen with anti-IgE or FMLP, but this was attributable to a lesser percent release in nonatopic subjects; atopic subjects responded similarly to all three agonists. MCAF was also shown to activate highly purified human basophils more readily than mixed leukocytes, and its activity was inhibited by a polyclonal rabbit antibody. At a suboptimal concentration (2.5 x 10(-9) M), MCAF was unable to prime the basophil to histamine release by other secretagogues. However, interleukin 3 (IL-3) and IL-5 could each prime basophils for MCAF-induced secretion. Therefore, our results suggest that MCAF may be a major contributor to the histamine-releasing activity seen in peripheral blood mononuclear cell supernatants that has been designated histamine releasing factor(s).This publication has 27 references indexed in Scilit:
- Properties of the Novel Proinflammatory Supergene "Intercrine" Cytokine FamilyAnnual Review of Immunology, 1991
- Interleukin 5 modifies histamine release and leukotriene generation by human basophils in response to diverse agonists.The Journal of Experimental Medicine, 1990
- Spontaneous Release of Histamine from Basophils and Histamine-Releasing Factor in Patients with Atopic Dermatitis and Food HypersensitivityNew England Journal of Medicine, 1989
- The effect of preseasonal immunotherapy on the production of histamine-refeasing factor (HRF) by mononuclear cells from patients with seasonal asthma: Results of a double-blind, placebo-controlled, randomized studyJournal of Allergy and Clinical Immunology, 1989
- Purification and further characterization of human mononuclear cell histamine-releasing factor.JCI Insight, 1989
- Identification of a histamine release inhibitory factor produced by human mononuclear cells in vitro.JCI Insight, 1988
- Human recombinant granulocyte-macrophage colony-stimulating factor and interleukin 3 cause basophil histamine release.JCI Insight, 1988
- Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor.The Journal of Experimental Medicine, 1988
- The magnitude of the spontaneous production of histamine-releasing factor (HRF) by lymphocytes in vitro correlates with the state of bronchial hyperreactivity in patients with asthmaJournal of Allergy and Clinical Immunology, 1987
- Characterization and functional studies of rheumatoid synovial mast cells: Activation by secretagogues, anti‐IgE, and a histamine‐releasing lymphokineArthritis & Rheumatism, 1986