Immunocytochemical studies of spectrin in hamster cardiac tissue
- 1 January 1989
- journal article
- research article
- Published by Wiley in Cell Motility
- Vol. 12 (3), 139-149
- https://doi.org/10.1002/cm.970120303
Abstract
The spectrins are a family of cytoskeletal-membrane proteins that have a wide tissue distribution. In the present study, we employed polyclonal antibodies made against mammalian and avian erythroid spectrins as well as mammalian brain spectrin to assess their presence and distributions in the mammalian heart. Western blot analyses revealed that all three antibodies were specific for a 240,000 molecular weight α-spectrin subunit found in hamster erythrocyte ghost homogenates, whole hamster heart, and isolated hamster cardiac myofibril homogenates. Spectrin staining was absent from the Triton X-100-extracted supernatant fraction of myofibril preparations, suggesting that the protein is linked to the myofibril precipitate after exposure to the detergent. Frozen, unfixed, 2-μm-thick; sections of adult, Syrian golden hamster cardiac tissue exhibited strong immunofluorescent staining of intercalated discs and Z-bands using all three antibodies. In addition, the mammalian erythroid spectrin antibodies showed staining of the sarcolemma, and in cross section, revealed a delicate internal network of staining that appears to surround individual myofibrils. This may be T-tubule-associated staining. Myofibrils isolated from cardiac myocytes using Triton X-100 show positive Z-band staining using all three antibodies. Double staining with Texas Red-labeled monoclonal desmin and FITC-labeled polyclonal spectrin antibodies revealed that both stained the myofibrillar Z-line regions. These results demonstrate that spectrin is closely associated with the membranes, myofibrils, and intermediate filaments in the mammalian heart.Keywords
This publication has 32 references indexed in Scilit:
- Spectrin and calmodulin in spreading mouse blastomeresDevelopmental Biology, 1988
- Cell type‐specific association between two types of spectrin and two types of intermediate filamentsCell Motility, 1987
- Brain spectrin(240/235) and brain spectrin(240/235E): two distinct spectrin subtypes with different locations within mammalian neural cells.The Journal of cell biology, 1986
- Association of spectrin with desmin intermediate filamentsJournal of Cellular Biochemistry, 1986
- Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1984
- Gamma actin, spectrin, and intermediate filament proteins colocalize with vinculin at costameres, myofibril‐to‐sarcolemma attachment sitesCell Motility, 1983
- Spectrin, fodrin, and TW260/240: A family of related proteins lining the plasma membraneCell Motility, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- ON THE STRUCTURAL CONTINUITIES OF THE TRANSVERSE TUBULAR SYSTEM OF RABBIT AND HUMAN MYOCARDIAL CELLSThe Journal of cell biology, 1963