Harvesting peripheral blood stem cells from healthy donors on 4th day of cytokine mobilization

Abstract

A pilot study was conducted to evaluate the efficacy of harvesting peripheral blood stem cells from normal healthy donors on day 4 after mobilization with G‐CSF at 10 μg/kg for 4 days or a sequential combination of GM‐CSF at 10 μg/kg for 2 days and G‐CSF at 10 μg/kg for 2 more days. Harvesting over the target dose (>4 × 10⁶ kg) of CD34+ cells based on the lst leukapheresis performed on day 4 was possible from 7 (53.8%) out of 13 donors. The 7 matched recipients all exhibited early engraftment, except for one who experienced transplant‐related mortality, and no differences were observed with the recipients transplanted with stem cells harvested after a 5–6‐day growth‐factor (GF) treatment. Accordingly, harvesting from normal healthy donors on day 4 after mobilization treatment with a GF was found to be feasible for allogeneic peripheral blood stem cell transplantation and more economical in terms of the cost of the GF and the donor's commitment. J. Clin. Apheresis 18:186–189, 2003.