A host dicer is required for defective viral RNA production and recombinant virus vector RNA instability for a positive sense RNA virus
Open Access
- 28 October 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 105 (43), 16749-16754
- https://doi.org/10.1073/pnas.0807225105
Abstract
Defective interfering (DI) RNAs, helper virus-dependent deletion mutant RNAs derived from the parental viral genomic RNA during replication, have been described for most RNA virus taxonomic groups. We now report that DI RNA production in the chestnut blight fungus, Cryphonectria parasitica, persistently infected by virulence-attenuating positive sense RNA hypoviruses, depends on one of two host dicer genes, dcl-2. We further report that nonviral sequences that are rapidly deleted from recombinant hypovirus RNA virus vectors in wild-type and dicer gene dcl-1 deletion mutant strains are stably maintained and expressed in the Δdcl-2 mutant strain. These results establish a requirement for dcl-2, the C. parasitica dicer gene responsible for antiviral defense and generation of virus-derived small interfering RNAs, in DI RNA production and recombinant virus vector RNA instability.Keywords
This publication has 39 references indexed in Scilit:
- Hypovirus Papain-Like Protease p48 Is Required for Initiation but Not for Maintenance of Virus RNA Propagation in the Chestnut Blight Fungus Cryphonectria parasiticaJournal of Virology, 2008
- Characterization of Hypovirus-Derived Small RNAs Generated in the Chestnut Blight Fungus by an Inducible DCL-2-Dependent PathwayJournal of Virology, 2008
- Specialization and evolution of endogenous small RNA pathwaysNature Reviews Genetics, 2007
- Antiviral Immunity Directed by Small RNAsCell, 2007
- Evidence that RNA silencing functions as an antiviral defense mechanism in fungiProceedings of the National Academy of Sciences of the United States of America, 2007
- Effects of Viral Strain, Transgene Position, and Target Cell Type on Replication Kinetics, Genomic Stability, and Transgene Expression of Replication-Competent Murine Leukemia Virus-Based VectorsJournal of Virology, 2007
- An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase geneJournal of General Virology, 2007
- Contribution of Protein p40 to Hypovirus-Mediated Modulation of Fungal Host Phenotype and Viral RNA AccumulationJournal of Virology, 2002
- Novel Design Architecture for Genetic Stability of Recombinant Poliovirus: the Manipulation of G/C Contents and Their Distribution Patterns Increases the Genetic Stability of Inserts in a Poliovirus-Based RPS-Vax Vector SystemJournal of Virology, 2002
- Initiation of polyribosome formation in poliovirus-infected HeLa cellsJournal of Molecular Biology, 1970