Different Fecal Microbiotas and Volatile Organic Compounds in Treated and Untreated Children with Celiac Disease
- 15 June 2009
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (12), 3963-3971
- https://doi.org/10.1128/aem.02793-08
Abstract
This study aimed at investigating the fecal microbiotas of children with celiac disease (CD) before (U-CD) and after (T-CD) they were fed a gluten-free diet and of healthy children (HC). Brothers or sisters of T-CD were enrolled as HC. Each group consisted of seven children. PCR-denaturing gradient gel electrophoresis (DGGE) analysis with V3 universal primers revealed a unique profile for each fecal sample. PCR-DGGE analysis with group- or genus-specific 16S rRNA gene primers showed that the Lactobacillus community of U-CD changed significantly, while the diversity of the Lactobacillus community of T-CD was quite comparable to that of HC. Compared to HC, the ratio of cultivable lactic acid bacteria and Bifidobacterium to Bacteroides and enterobacteria was lower in T-CD and even lower in U-CD. The percentages of strains identified as lactobacilli differed as follows: HC (ca. 38%) > T-CD (ca. 17%) > U-CD (ca. 10%). Lactobacillus brevis , Lactobacillus rossiae , and Lactobacillus pentosus were identified only in fecal samples from T-CD and HC. Lactobacillus fermentum , Lactobacillus delbrueckii subsp. bulgaricus , and Lactobacillus gasseri were identified only in several fecal samples from HC. Compared to HC, the composition of Bifidobacterium species of T-CD varied, and it varied even more for U-CD. Forty-seven volatile organic compounds (VOCs) belonging to different chemical classes were identified using gas-chromatography mass spectrometry-solid-phase microextraction analysis. The median concentrations varied markedly for HC, T-CD, and U-CD. Overall, the r 2 values for VOC data for brothers and sisters were equal to or lower than those for unrelated HC and T-CD. This study shows the effect of CD pathology on the fecal microbiotas of children.Keywords
This publication has 47 references indexed in Scilit:
- Imbalances in faecal and duodenal Bifidobacterium species composition in active and non-active coeliac diseaseBMC Microbiology, 2008
- Application of Sequence-Dependent Electrophoresis Fingerprinting in Exploring Biodiversity and Population Dynamics of Human Intestinal Microbiota: What Can Be Revealed?Interdisciplinary Perspectives on Infectious Diseases, 2008
- Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac childrenBMC Gastroenterology, 2008
- Bifidobacterium strains suppress in vitro the pro-inflammatory milieu triggered by the large intestinal microbiota of coeliac patientsJournal of Inflammation, 2008
- Advances in Celiac Disease and Gluten-Free DietJournal of the American Dietetic Association, 2008
- Live probioticBifidobacterium lactisbacteria inhibit the toxic effects induced by wheat gliadin in epithelial cell cultureClinical and Experimental Immunology, 2008
- Development of Bacteroides 16S rRNA Gene TaqMan-Based Real-Time PCR Assays for Estimation of Total, Human, and Bovine Fecal Pollution in WaterApplied and Environmental Microbiology, 2006
- VSL#3 probiotic preparation has the capacity to hydrolyze gliadin polypeptides responsible for Celiac Sprue probiotics and gluten intoleranceBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2006
- Influence of Resident Intestinal Microflora on the Development and Functions of the Gut-Associated Lymphoid TissueMicrobial Ecology in Health & Disease, 2001
- Rapid extraction of bacterial genomic DNA with guanidium thiocyanateLetters in Applied Microbiology, 1989