Isolation of material displaying insulin-like immunological biological activity from the brain of the blowfly Calliphora vomitoria
- 15 November 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 184 (2), 221-227
- https://doi.org/10.1042/bj1840221
Abstract
An insulin-like material from the brain of the blowfly Calliphora vomitoria was partially purified by acid alcohol extraction, gel filtration and ion-exchange cellulose chromatography. In addition, the RF value on polyacrylamide-gel electrophoresis was determined. The material was characterized by its ability to cross-react with bovine insulin antibody and by displaying diminished immunoreactivity on dilution. It displaced specifically bound 125I-labelled insulin from rat liver plasma membrane insulin receptors and displayed insulin-like biological activity on the isolated rat fat-cell. Within 30 min of injection into Calliphora, made hypertrehalocaemic and hyperglucaemic as a result of median neurosecretory cell removal, it caused the concentrations of both sugars to return to normal. The hypothesis is put forward that the median neurosecretory cells are the source of the material.This publication has 15 references indexed in Scilit:
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