Hematopoietic Progenitor Cells and Cellular Microenvironment: Behavioral and Molecular Changes upon Interaction
Open Access
- 1 September 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in The International Journal of Cell Cloning
- Vol. 23 (8), 1180-1191
- https://doi.org/10.1634/stemcells.2004-0361
Abstract
Cell–cell contact between stem cells and cellular determinants of the microenvironment plays an essential role in controlling cell division. Using human hematopoietic progenitor cells (CD34+/CD38−) and a stroma cell line (AFT024) as a model, we have studied the initial behavioral and molecular sequel of this interaction. Time‐lapse microscopy showed that CD34+/CD38− cells actively migrated toward and sought contact with stroma cells and 30% of them adhered firmly to AFT024 stroma through the uropod. CD44 and CD34 are colocalized at the site of contact. Gene expression profiles of CD34+/CD38− cells upon cultivation with or without stroma for 16, 20, 48, or 72 hours were analyzed using our human genome cDNA microarray. Chk1, egr1, and cxcl2 were among the first genes upregulated within 16 hours. Genes with the highest upregulation throughout the time course included tubulin genes, ezrin, c1qr1, fos, pcna, mcm6, ung, and dnmt1, genes that play an essential role in reorganization of the cytoskeleton system, stabilization of DNA, and methylation patterns. Our results demonstrate directed migration of CD34+/CD38− cells toward AFT024 and adhesion through the uropod and that upon interaction with supportive stroma, reorganization of the cytoskeleton system, regulation of cell division, and maintenance of genetic stability represent the most essential steps.Keywords
This publication has 63 references indexed in Scilit:
- Checking Out the CentrosomeCell Cycle, 2004
- Identification of the haematopoietic stem cell niche and control of the niche sizeNature, 2003
- Osteoblastic cells regulate the haematopoietic stem cell nicheNature, 2003
- Gene expression analysis of purified hematopoietic stem cells and committed progenitorsBlood, 2003
- Uracil in DNA – occurrence, consequences and repairOncogene, 2002
- A Stem Cell Molecular SignatureScience, 2002
- "Stemness": Transcriptional Profiling of Embryonic and Adult Stem CellsScience, 2002
- Comparison of gene expression in CD34+ cells from bone marrow and G-CSF-mobilized peripheral blood by high-density oligonucleotide array analysisTransplantation and Cellular Therapy, 2001
- Out of Eden: Stem Cells and Their NichesScience, 2000
- Conditions controlling the proliferation of haemopoietic stem cells in vitroJournal of Cellular Physiology, 1977