Formation and Decay of Prelumirhodopsin at Room Temperatures
- 1 October 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 69 (10), 2802-2806
- https://doi.org/10.1073/pnas.69.10.2802
Abstract
We have excited detergent-solubilized bovine rhodopsin at room temperature with 530-nm light pulses from a mode locked laser, and have observed the appearance and decay of a transient species that absorbs more strongly at 560 nm than does ground-state rhodopsin. Our data show that the absorbing intermediate appears in a time that is at least as short as the experimental resolution (about 6 psec) and decays with a life time of about 30 nsec. The extremely fast risetime supports the hypothesis that prelumirhodopsin is the product of the primary photoprocess.Keywords
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