A Toxoplasma Palmitoyl Acyl Transferase and the Palmitoylated Armadillo Repeat Protein TgARO Govern Apical Rhoptry Tethering and Reveal a Critical Role for the Rhoptries in Host Cell Invasion but Not Egress

Abstract

Apicomplexans are obligate intracellular parasites that actively penetrate their host cells to create an intracellular niche for replication. Commitment to invasion is thought to be mediated by the rhoptries, specialized apical secretory organelles that inject a protein complex into the host cell to form a tight-junction for parasite entry. Little is known about the molecular factors that govern rhoptry biogenesis, their subcellular organization at the apical end of the parasite and subsequent release of this organelle during invasion. We have identified a Toxoplasma palmitoyl acyltransferase, TgDHHC7, which localizes to the rhoptries. Strikingly, conditional knockdown of TgDHHC7 results in dispersed rhoptries that fail to organize at the apical end of the parasite and are instead scattered throughout the cell. While the morphology and content of these rhoptries appears normal, failure to tether at the apex results in a complete block in host cell invasion. In contrast, attachment and egress are unaffected in the knockdown, demonstrating that the rhoptries are not required for these processes. We show that rhoptry targeting of TgDHHC7 requires a short, highly conserved C-terminal region while a large, divergent N-terminal domain is dispensable for both targeting and function. Additionally, a point mutant lacking a key residue predicted to be critical for enzyme activity fails to rescue apical rhoptry tethering, strongly suggesting that tethering of the organelle is dependent upon TgDHHC7 palmitoylation activity. We tie the importance of this activity to the palmitoylated Armadillo Repeats-Only (TgARO) rhoptry protein by showing that conditional knockdown of TgARO recapitulates the dispersed rhoptry phenotype of TgDHHC7 knockdown. The unexpected finding that apicomplexans have exploited protein palmitoylation for apical organelle tethering yields new insight into the biogenesis and function of rhoptries and may provide new avenues for therapeutic intervention against Toxoplasma and related apicomplexan parasites. Apicomplexans possess a highly polarized secretory pathway that is critical for their ability to invade host cells and cause disease. This unique cellular organization enables delivery of protein cargo to specialized secretory organelles called micronemes and rhoptries that drive forward penetration into the host cell. The rhoptries are tethered in a bundle at the apex of the parasite, but how these organelles are organized in this manner is unknown. In this work, we identify a rhoptry-localized palmitoyl acyl transferase (named TgDHHC7) that functions to properly affix the rhoptries at the apical end of the parasite. Conditional disruption of TgDHHC7 results in a failure to tether the rhoptries at the cell apex and a corresponding loss of rhoptry function. We exploit this mutant to clearly demonstrate a critical role for the rhoptries in host invasion but not attachment or egress. Additionally, we find that mutation of a key residue predicted to be required for catalytic activity renders TgDHHC7 non-functional and that knockdown of the candidate substrate TgARO produces an identical phenotype to loss of TgDHHC7. The finding that Toxoplasma employs protein palmitoylation to position the rhoptries at the cell apex provides new insight into the molecular mechanisms that underlie apicomplexan cell polarity, host invasion and pathogenesis.