Optimization of the touchscreen paired-associate learning (PAL) task for mice and its dorsal hippocampal dependency
- 23 August 2016
- journal article
- research article
- Published by Taylor & Francis Ltd in Animal Cells and Systems
- Vol. 20 (5), 229-236
- https://doi.org/10.1080/19768354.2016.1221855
Abstract
Two versions of the touchscreen paired-associate learning (PAL) task have been developed for rodents: same PAL (sPAL) and different PAL (dPAL). These tasks are very important in studying murine models of Alzheimer’s disease and schizophrenia, and have also been used to test object-location memory in various studies. However, the relatively long time needed for the tasks (approx. 50 days for mice) limits their widespread use. By giving training that was more intensive with a higher number of trials, we shortened the time required for learning saturation in sPAL and dPAL to about one-third of the time required for the generally used protocol. Furthermore, by applying a reduced number of objects and trial types for sPAL, we developed a simplified version of sPAL, termed 2-object sPAL, in which mice could reach the fully learned level in 6 days. Our pharmacological experiments indicate that the dorsal hippocampal CA1 region is crucial for the performance of the two PAL tasks with the new protocols and the new 2-object sPAL. This work has significantly enhanced the usefulness of the touchscreen PAL tasks to increase the speed of learning, but they remain highly hippocampus-dependent object-location memory tasks.Keywords
Funding Information
- National Honor Scientist Program of Korea (2012R1A3A1050385)
This publication has 25 references indexed in Scilit:
- New translational assays for preclinical modelling of cognition in schizophrenia: The touchscreen testing method for mice and ratsNeuropharmacology, 2012
- Dynamics of Retrieval Strategies for Remote MemoriesCell, 2011
- A Functional Role for Adult Hippocampal Neurogenesis in Spatial Pattern SeparationScience, 2009
- The touchscreen cognitive testing method for rodents: How to get the best out of your ratLearning & Memory, 2008
- Pharmacological Manipulation of Neuronal Ensemble Activity by Reverse Microdialysis in Freely Moving Rats: A Comparative Study of the Effects of Tetrodotoxin, Lidocaine, and MuscimolThe Journal of pharmacology and experimental therapeutics, 2007
- Morris water maze: procedures for assessing spatial and related forms of learning and memoryNature Protocols, 2006
- The effect of overlearning on long‐term retentionApplied Cognitive Psychology, 2004
- Applications of the Morris water maze in the study of learning and memoryBrain Research Reviews, 2001
- Early Detection and Differential Diagnosis of Alzheimer’s Disease and Depression with Neuropsychological TasksDementia and Geriatric Cognitive Disorders, 2001
- Effect of overlearning on retention.Journal of Applied Psychology, 1992