A METHOD FOR THE DETERMINATION OF 17-HYDROXYCORTICOSTEROIDS IN BLOOD: 17-HYDROXYCORTICOSTERONE IN THE PERIPHERAL CIRCULATION*

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Abstract
A method is described for the quantitative determination of adrenal steroids in peripheral blood. The method consists essentially of ether-chloroform extraction, partitioning of the ether-chloroform soluble material between 70% ethanol and hexane, and chromatography of the ethanol-soluble fraction on a magnesium silicate-Celite or Florosil column. Quantitative determination of the 17-hydroxycortico-steroids is carried out by the use of a micro modification of the color reaction described by Porter and Silber. Normal values for 17-hydroxycorticosteroids in human peripheral blood ranged between 4 and 10 [mu]g./100 ml. of whole blood.