Ultrastructural Effects of Lactoferrin Binding on Giardia lamblia Trophozoites
- 1 January 1997
- journal article
- Published by Wiley in The Journal of Eukaryotic Microbiology
- Vol. 44 (1), 68-72
- https://doi.org/10.1111/j.1550-7408.1997.tb05694.x
Abstract
Lactoferrin and its derived N‐terminal peptide may be important host defenses against Giardia lamblia. We showed earlier that lactoferrin and the derived peptides have potent giardicidal activity in vitro. Using indirect immunofluorescence, we now demonstrate binding of lactoferrin and the peptides to the live trophozoite surface. Iron strongly inhibited binding of lactoferrin, and decreased binding of the peptides, while certain divalent metal ions decreased binding of all forms by about half. Lactoferrin and the peptedes caused striking and complex morphologic changes in the trophozoite plasmalemma, endomembranes and cytoskeleton, and increases the electron density of the lysosome‐like peripheral vacuoles.Keywords
This publication has 25 references indexed in Scilit:
- A review: The active peptide of lactoferrinPediatrics International, 1994
- Antibacterial spectrum of lactoferricin B, a potent bactericidal peptide derived from the N‐terminal region of bovine lactoferrinJournal of Applied Bacteriology, 1992
- Identification of the bactericidal domain of lactoferrinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
- Killing of gram-negative bacteria by lactoferrin and lysozyme.JCI Insight, 1991
- Killing of Giardia lamblia Trophozoites by Human Intestinal Fluid in VitroThe Journal of Infectious Diseases, 1988
- Lactoferrin in human milk: its role in iron absorption and protection against enteric infection in the newborn infant.Archives of Disease in Childhood, 1980
- Distribution of transferrin, ferritin, and lactoferrin in human tissues.Journal of Clinical Pathology, 1978
- A Bactericidal Effect for Human LactoferrinScience, 1977
- LACTOFERRIN AND IRON ABSORPTION IN THE SMALL INTESTINEActa Medica Scandinavica, 1974
- An iron-binding protein common to many external secretionsClinica Chimica Acta; International Journal of Clinical Chemistry, 1966