Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing
Top Cited Papers
Open Access
- 17 February 2004
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 10 (3), 544-550
- https://doi.org/10.1261/rna.5235104
Abstract
A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2′-O-methyl oligoribonucleotides, but not 2′-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA–protein complexes in human cell extracts as well as in cultured human cells.Keywords
This publication has 74 references indexed in Scilit:
- A microRNA array reveals extensive regulation of microRNAs during brain developmentRNA, 2003
- Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genesNucleic Acids Research, 2003
- The Small RNA Profile during Drosophila melanogaster DevelopmentDevelopmental Cell, 2003
- Control of leaf morphogenesis by microRNAsNature, 2003
- Heterochromatin and Epigenetic Control of Gene ExpressionScience, 2003
- MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanismsProceedings of the National Academy of Sciences of the United States of America, 2003
- Embryonic Stem Cell-Specific MicroRNAsDevelopmental Cell, 2003
- Role of MicroRNAs in Plant and Animal DevelopmentScience, 2003
- Computational identification of DrosophilamicroRNA genesGenome Biology, 2003
- Towards a complete description of the microRNA complement of animal genomesGenome Biology, 2003