Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus
Open Access
- 9 January 2019
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 20 (2), 246
- https://doi.org/10.3390/ijms20020246
Abstract
The Kv4 family of voltage-gated K+ channels underlie the fast transient (A-type) outward K+ current. Although A-type currents are critical to determine somato-dendritic integration in central neurons, relatively little is known about the precise subcellular localisation of the underlying channels in hippocampal circuits. Using histoblot and immunoelectron microscopic techniques, we investigated the expression, regional distribution and subcellular localisation of Kv4.2 and Kv4.3 in the adult brain, as well as the ontogeny of their expression during postnatal development. Histoblot demonstrated that Kv4.2 and Kv4.3 proteins were widely expressed in the brain, with mostly non-overlapping patterns. During development, levels of Kv4.2 and Kv4.3 increased with age but showed marked region- and developmental stage-specific differences. Immunoelectron microscopy showed that labelling for Kv4.2 and Kv4.3 was differentially present in somato-dendritic domains of hippocampal principal cells and interneurons, including the synaptic specialisation. Quantitative analyses indicated that most immunoparticles for Kv4.2 and Kv4.3 were associated with the plasma membrane in dendritic spines and shafts, and that the two channels showed very similar distribution patterns in spines of principal cells and along the surface of granule cells. Our data shed new light on the subcellular localisation of Kv4 channels and provide evidence for their non-uniform distribution over the plasma membrane of hippocampal neurons.Funding Information
- Ministerio de Ciencia y Tecnología (BFU2015-63769-R)
- Horizon 2020 (720270)
This publication has 44 references indexed in Scilit:
- Subcellular Localization of K+ Channels in Mammalian Brain Neurons: Remarkable Precision in the Midst of Extraordinary ComplexityNeuron, 2015
- Dendritic ion channelopathy in acquired epilepsyEpilepsia, 2012
- Nonselective Cation Channels and Links to Hippocampal Ischemia, Aging, and DementiaAdvances in Experimental Medicine and Biology, 2012
- Molecular physiology and modulation of somatodendritic A-type potassium channelsMolecular and Cellular Neuroscience, 2004
- Structure and Function of Kv4-Family Transient Potassium ChannelsPhysiological Reviews, 2004
- The CD26-Related Dipeptidyl Aminopeptidase-like Protein DPPX Is a Critical Component of Neuronal A-Type K+ ChannelsNeuron, 2003
- Molecular Cloning and Characterization of CALP/KChIP4, a Novel EF-hand Protein Interacting with Presenilin 2 and Voltage-gated Potassium Channel Subunit Kv4Online Journal of Public Health Informatics, 2002
- Elimination of fast inactivation in Kv4 A-type potassium channels by an auxiliary subunit domainProceedings of the National Academy of Sciences of the United States of America, 2002
- Modulation of A-type potassium channels by a family of calcium sensorsNature, 2000
- Calsenilin: A calcium-binding protein that interacts with the presenilins and regulates the levels of a presenilin fragmentNature Medicine, 1998