Inhibition of human intestinalα-glucosidehydrolases by a new complex oligosaccharide
- 1 February 1979
- journal article
- research article
- Published by Springer Science and Business Media LLC in Zeitschrift für Die Gesamte Experimentelle Medizin
- Vol. 175 (1), 1-6
- https://doi.org/10.1007/bf01851228
Abstract
The effect of a new complex oligosaccharide (Bay g 5421) of microbial origin on human intestinalα-glucosidehydrolase activity was tested in mucosal homogenate from human small bowel biopsy specimens. Theα-glucosidehydrolase inhibitor (α-GHI) exerted a potent inhibitory effect on glucoamylase, sucrase, and maltase, was minimally effective on isomaltase, and did not affect trehalase and lactase activity. Kinetic analysis revealed a fully competitive type of inhibition with a Ki of 1.3 × 10−6M; thus the inhibitor had a 15 000-fold higher affinity to the enzyme sucrase than its natural substrate sucrose. The new compound may prove to be useful in the study of carbohydrate maldigestion and malabsorption and may possibly be of therapeutic benefit in diabetes and obesity.This publication has 11 references indexed in Scilit:
- Glucosidase inhibitionThe Science of Nature, 1977
- ?-Glucosidase inhibitorsThe Science of Nature, 1977
- Sucrase-Isomaltase DeficiencyNew England Journal of Medicine, 1976
- Carbohydrate Digestion and AbsorptionNew England Journal of Medicine, 1975
- Inhibition of sucrase by tris in rat and man, demonstrated by oral loading tests with sucroseMetabolism, 1975
- Untersuchungen zum Einfluß von Prenylamin auf die Dünndarmresorption beim Menschen*Deutsche Medizinische Wochenschrift (1946), 1974
- Purification of the human intestinal brush border membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973
- Influence of an ??-amylase inhibitor (BAY d 7791) on blood glucose, serum insulin and nefa in starch loading tests in rats, dogs and manDiabetologia, 1973
- Method for assay of intestinal disaccharidasesAnalytical Biochemistry, 1964
- Phlorizin as a competitive inhibitor of the active transport of sugars by hamster small intestine, in vitroBiochimica et Biophysica Acta, 1962