Produced Water Exposure Alters Bacterial Response to Biocides
- 22 October 2014
- journal article
- research article
- Published by American Chemical Society (ACS) in Environmental Science & Technology
- Vol. 48 (21), 13001-13009
- https://doi.org/10.1021/es5036915
Abstract
Microbial activity during the holding and reuse of wastewater from hydraulic fracturing operations, termed produced water, may lead to issues with corrosion, sulfide release, and fouling. Biocides are applied to control biological activity, often with limited efficacy, which is typically attributed to chemical interactions with the produced water. However, it is unknown whether there is a biologically driven mechanism to biocide tolerance in produced water. Here, we demonstrate that produced water exposure results in an enhanced tolerance against the typically used biocide glutaraldehyde and increased susceptibility to the oxidative biocide hypochlorite in a native and a model bacteria and that this altered resistance is due to the salinity of the produced water. In addition, we elucidate the genetic response of the model organism Pseudomonas fluorescens to produced water exposure to provide a mechanistic interpretation of the altered biocide resistance. The RNA-seq data demonstrated the induction of genes involved in osmotic stress, energy production and conversion, membrane integrity, and protein transport following produced water exposure, which facilitates bacterial survival and alters biocide tolerance. Efforts to fundamentally understand biocide resistance mechanisms, which enable the optimization of biocide application, hold significant implications for greening of the fracturing process through encouraging produced water recycling. Specifically, these results suggest the necessity of optimizing biocide application at the level of individual shale plays, rather than historical experience, based upon produced water characteristics and salinity.Keywords
Funding Information
- U.S. Department of Energy - National Energy Technology Laboratory (409880)
This publication has 53 references indexed in Scilit:
- Directional RNA-seq reveals highly complex condition-dependent transcriptomes in E. coli K12 through accurate full-length transcripts assemblingBMC Genomics, 2013
- RNA-seq analysis of single bovine blastocystsBMC Genomics, 2013
- High-Salinity Growth Conditions Promote Tat-Independent Secretion of Tat Substrates in Bacillus subtilisApplied and Environmental Microbiology, 2012
- Differential expression in RNA-seq: A matter of depthGenome Research, 2011
- Deep RNA sequencing of L. monocytogenes reveals overlapping and extensive stationary phase and sigma B-dependent transcriptomes, including multiple highly transcribed noncoding RNAsBMC Genomics, 2009
- RNA-Seq: a revolutionary tool for transcriptomicsNature Reviews Genetics, 2009
- Systematic and integrative analysis of large gene lists using DAVID bioinformatics resourcesNature Protocols, 2008
- Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene listsNucleic Acids Research, 2008
- Mapping and quantifying mammalian transcriptomes by RNA-SeqNature Methods, 2008
- GroES/GroEL and DnaK/DnaJ Have Distinct Roles in Stress Responses and during Cell Cycle Progression inCaulobacter crescentusJournal of Bacteriology, 2006