A Liquid Chromatographic Assay for 5‐Hydroxytryptophan, Serotonin and 5‐Hydroxyindoleacetic Acid in Human Body Fluids
- 1 November 1982
- journal article
- Published by Wiley in Acta Pharmacologica et Toxicologica
- Vol. 51 (5), 421-427
- https://doi.org/10.1111/j.1600-0773.1982.tb01047.x
Abstract
We describe the precision and accuracy of a liquid chromatographic method, which uses internal standards, 6-fluoroserotonin and 5-hydroxyindolecarboxylic acid, in quantitating serotonin and 5-hydroxyindoleacetic acid in human cerebrospinal fluid, plasma and urine. In addition, 5-hydroxytryptophan is measured in the cerebrospinal fluid. The limit of sensitivity of this method is 0.1 pmol/injection, the peak height/concentration ratio is linear in the concentration range of 0.1 pmol to 50 mumol/injection, and the coefficient of variation is of the order of 15% at the limit of sensitivity and below 10% at amounts above 0.5 pmol/injection. No endogenous monoamines or their metabolites interfere with the quantitation of the substances of interest in the body fluids studied.Keywords
This publication has 20 references indexed in Scilit:
- Application of high performance liquid chromatography with electrochemical detection to neurochemical analysis: measurement of catecholamines, serotonin and metabolites in rat brainJournal of Neuroscience Methods, 1981
- Simultaneous determination of femtomole quantities of 5-hydroxytryptophan, serotonin and 5-hydroxyindoleacetic acid in brain using HPLC with electrochemical detectionEuropean Journal of Pharmacology, 1981
- I. Liquid chromatography with pre-column sample enrichment and electrochemical detection. Regional determination of serotonin and 5-hydroxyindoleacetic acid in brain tissueLife Sciences, 1980
- Determination of serotonin in serum and plasma by liquid chromatography with precolumn sample enrichment and electrochemical detectionAnalytical Chemistry, 1980
- Aggression in humans correlates with cerebrospinal fluid amine metabolitesPsychiatry Research, 1979
- Determination of picogram levels of brain serotonin by a simplified liquid chromatographic electrochemical detection assayBrain Research Bulletin, 1979
- Liquid chromatographic-fluorometric system for the determination of indoles in physiological samplesAnalytical Chemistry, 1979
- Synthesis of ring‐fluorinated serotonins and melatoninsJournal of Heterocyclic Chemistry, 1976
- "Serotonin Depression"—A Biochemical Subgroup Within the Affective Disorders?Science, 1976
- Quantitative determination of 5-hydroxyindole-3-acetic acid in cerebrospinal fluid by gas chromatography-mass spectrometryAnalytical Chemistry, 1972