pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi
- 1 March 1994
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 11 (6), 1127-1139
- https://doi.org/10.1111/j.1365-2958.1994.tb00389.x
Abstract
Erwinia chrysanthemi mutants (designated as pecS) displaying derepressed pectate lyase and cellulase synthesis were isolated. In addition, the pecS mutation is responsible for production of an extracellular insoluble blue pigment whose synthesis is cryptic in the wild-type 3937 strain. Transduction analysis indicates that the phenotype is due to a single mutation located near the xyl marker on the strain 3937 chromosome. This mutation was complemented by an R-prime plasmid carrying the xyl and argG genes of E. chrysanthemi, suggesting that the pecS product acts in trans to modulate pectinase, cellulase and blue pigment production. Insertion mutagenesis of the cloned region and recombination of the corresponding mutations in the bacterial chromosome by marker exchange revealed the existence of two divergently transcribed genes, pecS and pecM, that are both involved in the pectate lyase and cellulase regulation. The nucleotide sequences of pecS and pecM were determined. The pecS gene encodes a 166 amino acid polypeptide that shows similarity to the MprA regulatory protein of Escherichia coli whereas the pecM gene encodes a 297 amino acid polypeptide that was shown to be an integral membrane protein. The possible functions of the PecS and PecM proteins derived from the mutant phenotype and sequence analysis are discussed in terms of signal transduction and transcription regulation.Keywords
This publication has 62 references indexed in Scilit:
- Specific Interactions of Erwinia chrysanthemi KdgR Repressor with Different Operators of Genes Involved in PectinolysisJournal of Molecular Biology, 1994
- Regulation of the expression of a pelA:: uidA fusion in Erwinia chrysanthemi and demonstration of the synergistic action of plant extract with polygalacturonate on pectate lyase synthesisJournal of General Microbiology, 1993
- Characterization of a Novel Regulatory GeneaepAthat Controls Extracellular Enzyme Production in the Phytopathogenic BacteriumErwinia carotovorasubsp.carotovoraMolecular Plant-Microbe Interactions®, 1993
- Small molecule-mediated density-dependent control of gene expression in prokaryotes: Bioluminescence and the biosynthesis of carbapenem antibioticsFEMS Microbiology Letters, 1992
- Isorepressor of the gal regulon in Escherichia coliJournal of Molecular Biology, 1992
- TRANSCRIPTION FACTORS: Structural Families and Principles of DNA RecognitionAnnual Review of Biochemistry, 1992
- ′uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructionsFEMS Microbiology Letters, 1992
- Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transferGene, 1991
- Nucleotide sequences of the Erwinia chrysanthemi ogl and pelE genes negatively regulated by the kdgR gene productGene, 1989
- Tn5insertion inkdgR, a regulatory gene of the polygalacturonate pathway inErwinia chrysanthemiFEMS Microbiology Letters, 1987