Gene Expression Biomarkers Provide Sensitive Indicators of in Planta Nitrogen Status in Maize
Open Access
- 6 October 2011
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 157 (4), 1841-1852
- https://doi.org/10.1104/pp.111.187898
Abstract
Over the last several decades, increased agricultural production has been driven by improved agronomic practices and a dramatic increase in the use of nitrogen-containing fertilizers to maximize the yield potential of crops. To reduce input costs and to minimize the potential environmental impacts of nitrogen fertilizer that has been used to optimize yield, an increased understanding of the molecular responses to nitrogen under field conditions is critical for our ability to further improve agricultural sustainability. Using maize (Zea mays) as a model, we have characterized the transcriptional response of plants grown under limiting and sufficient nitrogen conditions and during the recovery of nitrogen-starved plants. We show that a large percentage (approximately 7%) of the maize transcriptome is nitrogen responsive, similar to previous observations in other plant species. Furthermore, we have used statistical approaches to identify a small set of genes whose expression profiles can quantitatively assess the response of plants to varying nitrogen conditions. Using a composite gene expression scoring system, this single set of biomarker genes can accurately assess nitrogen responses independently of genotype, developmental stage, tissue type, or environment, including in plants grown under controlled environments or in the field. Importantly, the biomarker composite expression response is much more rapid and quantitative than phenotypic observations. Consequently, we have successfully used these biomarkers to monitor nitrogen status in real-time assays of field-grown maize plants under typical production conditions. Our results suggest that biomarkers have the potential to be used as agronomic tools to monitor and optimize nitrogen fertilizer usage to help achieve maximal crop yields.Keywords
This publication has 32 references indexed in Scilit:
- Functional Analysis of the Arabidopsis PAL Gene Family in Plant Growth, Development, and Response to Environmental StressPlant Physiology, 2010
- A system biology approach highlights a hormonal enhancer effect on regulation of genes in a nitrate responsive "biomodule"BMC Systems Biology, 2009
- MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in ArabidopsisTHE PLANT CELL ONLINE, 2009
- Increasing Crop Productivity to Meet Global Needs for Feed, Food, and FuelPlant Physiology, 2009
- Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1Proceedings of the National Academy of Sciences of the United States of America, 2008
- Marker-assisted selection: an approach for precision plant breeding in the twenty-first centuryPhilosophical Transactions B, 2007
- Global transcription profiling reveals differential responses to chronic nitrogen stress and putative nitrogen regulatory components in ArabidopsisBMC Genomics, 2007
- TheArabidopsisTranscription Factor MYB77 Modulates Auxin Signal TransductionTHE PLANT CELL ONLINE, 2007
- Expression Profiles of 10,422 Genes at Early Stage of Low Nitrogen Stress in Rice Assayed using a cDNA MicroarrayPublished by Springer Science and Business Media LLC ,2006
- Gene Expression Omnibus: NCBI gene expression and hybridization array data repositoryNucleic Acids Research, 2002