Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success
- 1 March 2000
- journal article
- research article
- Published by Hindawi Limited in Aquaculture Research
- Vol. 31 (3), 289-296
- https://doi.org/10.1046/j.1365-2109.2000.00400.x
Abstract
Collection of fish milt by stripping risks the danger of milt contamination by urine. This may seriously influence milt characteristics and quality, including usefulness for cryopreservation. Urine contamination of milt may be avoided by using a catheter for sperm collection. The objectives of this study were to provide basic characteristics of milt collected with a catheter, to test the usefulness of this milt for cryopreservation, and to correlate characteristics of fresh and cryopreserved semen with sperm fertility rates. Milt from 25 rainbow trout Oncorhynchus mykiss (Walbaum) males were used. All samples were cryopreserved using the pellet method within 1 h of collection, using 0.6 m sucrose and 10% dimethyl sulphoxide (DMSO) as an extender. Catheterization resulted in semen of very good motility (> 90% motile spermatozoa) and high fertilization rates after cryopreservation (mean fertilization rate 81.8 ± 13.3% of control, at a sperm/egg ratio of 2.4 ± 0.3 × 106). Osmolality of seminal plasma and concentrations of sodium, potassium and magnesium ions had low variability, which suggests that they are important for creating a stable environment for sperm storage in the sperm duct. Higher variability of certain seminal plasma characteristics, such as protein concentration and antiproteinase activity, suggests that these characteristics are related to individual semen features of particular males. A strong correlation of seminal plasma zinc concentration with protein concentration may reflect an importance of zinc in semen biology. Cryopreservation caused a significant release of protein and acid phosphatase from spermatozoa. Our results did not reveal any single characteristic of semen collected by catheter that could be used as a powerful predictor of cryopreservation success, presumably because all samples were of high quality.This publication has 42 references indexed in Scilit:
- Effect of urine on semen quality in turbot (Psetta maxima)Aquaculture, 1998
- Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolismAquaculture, 1998
- Initiation of carp spermatozoa motility and early ATP reduction after milt contamination by urineAquaculture, 1998
- Cryopreservation of rainbow trout (Oncorhynchus mykiss) spermatozoa using programmable freezingAquaculture, 1996
- Cryopreservation of rainbow trout (Oncorhynchus mykiss) sperm: practical recommendationsAquaculture, 1993
- Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric techniqueAquaculture, 1993
- Reproduction in rainbow trout: sex differentiation, dynamics of gametogenesis, biology and preservation of gametesAquaculture, 1992
- Seasonal changes in the quality of rainbow trout (Salmo gairdneri) semen: Effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constituentsAquaculture, 1987
- Cryopreservation of rainbow trout (Salmo gairdneri) spermAquaculture, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970