Distinguishing angioimmunoblastic T‐cell lymphoma from peripheral T‐cell lymphoma, unspecified, using morphology, immunophenotype and molecular genetics
- 28 February 2007
- journal article
- Published by Wiley in Histopathology
- Vol. 50 (4), 498-508
- https://doi.org/10.1111/j.1365-2559.2007.02632.x
Abstract
To identify distinguishing histological, immunophenotypic and molecular genetic features between angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma (PTL). Nodal T-cell lymphomas examined (n =137), included AITL (n = 89), PTL (n = 22), anaplastic large cell lymphoma (n = 16) and 'AITL/PTL indeterminate' (n = 10) with overlapping features between AITL and PTL, showing morphology typical of AITL but lacking follicular dendritic cell expansion. Immunohistochemistry for CD3, CD20, CD21 and CD10, in situ hybridization for Epstein-Barr virus encoded RNA (EBER) and polymerase chain reaction for T-cell and B-cell clonality analysis were performed. Of the AITLs, 74/89 showed typical morphology, whereas 15/89 showed hyperplastic follicles. AITL and 'AITL/PTL indeterminate' showed a polymorphous infiltrate and prominent vascularity in all cases. In both groups, CD10 was present in the majority and clear cells and EBER positivity were specific (but not universal) features lacking in PTL. Detection of T-cell clonality was significantly higher in AITL (90%) compared with PTLu (59%). Clear cells and EBV infection (when present) are useful distinguishing features and CD10 a sensitive and specific marker of AITL. Hyperplastic follicles are present in a significant minority of AITL. AITL/PTL indeterminate probably falls within the spectrum of AITL rather than PTL.Keywords
This publication has 27 references indexed in Scilit:
- CD10 expression in peripheral T-cell lymphomas complicated by a proliferation of large B-cellsLaboratory Investigation, 2006
- Novel t(5;9)(q33;q22) fuses ITK to SYK in unspecified peripheral T-cell lymphomaLeukemia, 2005
- Epstein-Barr Virus–Associated B-Cell Lymphoproliferative Disorders in Angioimmunoblastic T-Cell Lymphoma and Peripheral T-Cell Lymphoma, UnspecifiedAmerican Journal of Clinical Pathology, 2002
- Neoplastic T cells in angioimmunoblastic T-cell lymphoma express CD10Blood, 2002
- Kaposi sarcoma-associated herpesvirus infects monotypic (IgMlambda) but polyclonal naive B cells in Castleman disease and associated lymphoproliferative disordersBlood, 2001
- Peripheral T-Cell Lymphoma Complicated by a Proliferation of Large B CellsAmerican Journal of Clinical Pathology, 2000
- Peripheral T-Cell Lymphoma With Distinct Perifollicular Growth PatternThe American Journal of Surgical Pathology, 2000
- Paracortical Nodular T-Cell LymphomaThe American Journal of Surgical Pathology, 1995
- Common acute lymphobiastic leukemia antigen (CD10)‐positive Sézary's syndromeAmerican Journal of Hematology, 1994
- Detection of monoclonality in low‐grade B‐cell lymphomas using the polymerase chain reaction is dependent on primer selection and lymphoma typeThe Journal of Pathology, 1993