Single amino acid substitution between SHV‐1 β‐lactamase and cefotaxime‐hydrolyzing SHV‐2 enzyme
- 11 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (1), 217-220
- https://doi.org/10.1016/0014-5793(88)80734-8
Abstract
SHV-2 β-lactamase was purified from an overproducing variant of a clinical isolate of Escherichia coli resistant to cefotaxime. Pure protein was digested by trypsin and Lys-C endoproteinase. Proteolytic peptides, isolated by reverse-phase HPLC, were submitted to manual Edman degradation and aligned by homology with the sequence of SHV-1 β-lactamase. A putative amino acid sequence was deduced. Structural comparison revealed that SHV-2 differed from SHV-1 by only one amino acid, Gly → Ser, at position 213 of the mature protein.This publication has 13 references indexed in Scilit:
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