VASCULAR‐RELATED NAC‐DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation
Open Access
- 1 February 2011
- journal article
- Published by Wiley in The Plant Journal
- Vol. 66 (4), 579-590
- https://doi.org/10.1111/j.1365-313x.2011.04514.x
Abstract
The Arabidopsis thaliana NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), acts as a key regulator of xylem vessel differentiation. In order to identify direct target genes of VND7, we performed global transcriptome analysis using Arabidopsis transgenic lines in which VND7 activity could be induced post‐translationally. This analysis identified 63 putative direct target genes of VND7, which encode a broad range of proteins, such as transcription factors, IRREGULAR XYLEM proteins and proteolytic enzymes, known to be closely associated with xylem vessel formation. Recombinant VND7 protein binds to several promoter sequences present in candidate direct target genes: specifically, in the promoter of XYLEM CYSTEINE PEPTIDASE1, two distinct regions were demonstrated to be responsible for VND7 binding. We also found that expression of VND7 restores secondary cell wall formation in the fiber cells of inflorescence stems of nst1 nst3 double mutants, as well as expression of NAC SECONDARY WALL THICKENING PROMOTING FACTOR3 (NST3, however, the vessel‐type secondary wall deposition was observed only as a result of VND7 expression. These findings indicated that VND7 upregulates, directly and/or indirectly, many genes involved in a wide range of processes in xylem vessel differentiation, and that its target genes are partially different from those of NSTs.Keywords
This publication has 34 references indexed in Scilit:
- Regulatory Mechanisms for Specification and Patterning of Plant Vascular TissuesAnnual Review of Cell and Developmental Biology, 2010
- Regulation of plant biomass productionCurrent Opinion in Plant Biology, 2010
- Ectopic expression of MYB46 identifies transcriptional regulatory genes involved in secondary wall biosynthesis in ArabidopsisThe Plant Journal, 2009
- Cysteine proteases XCP1 and XCP2 aid micro‐autolysis within the intact central vacuole during xylogenesis in Arabidopsis rootsThe Plant Journal, 2008
- ANAC012, a member of the plant‐specific NAC transcription factor family, negatively regulates xylary fiber development in Arabidopsis thalianaThe Plant Journal, 2007
- Identification of Novel Genes in Arabidopsis Involved in Secondary Cell Wall Formation Using Expression Profiling and Reverse GeneticsPlant Cell, 2005
- The homeotic protein AGAMOUS controls microsporogenesis by regulation of SPOROCYTELESSNature, 2004
- Signals that control plant vascular cell differentiationNature Reviews Molecular Cell Biology, 2004
- The Arabidopsis Xylem Peptidase XCP1 Is a Tracheary Element Vacuolar Protein That May Be a Papain OrthologPlant Physiology, 2002
- A glucocorticoid‐mediated transcriptional induction system in transgenic plantsThe Plant Journal, 1997