Characterization of a Soluble NADH-Independent Nitrate Reductase from the Photosynthetic Bacterium Rhodopseudomonas capsulata

Abstract

The assimilatory nitrate reductase was purified 60-fold from a newly isolated, nitrate assimilating strain of the photosynthetic bacterium R. capsulata. The enzyme had a MW of about 180,000 daltons and was typically prokaryotic in that it was not active with reduced pyridine nucleotides but rather with reduced flavins.