Open sandwich ELISA: A novel immunoassay based on the interchain interaction of antibody variable region
- 1 December 1996
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 14 (13), 1714-1718
- https://doi.org/10.1038/nbt1296-1714
Abstract
We describe an immunoassay that is based on the interchain interaction of separated VL and VH chains from a single chain antibody variable region. In the presence of antigen, the chains reassociate. VL fragments of anti-hen egg lysozyme (HEL) antibody HyHEL-10 were immobilized on microtiter plates. Samples were coincubated with an M13-displayed VH chain, and assayed with peroxidase-labeled anti-M13 antibody. Signal was detected in direct proportion to the amount of HEL in the sample. Wide dynamic range with <15 ng/ml sensitivity was attained.Keywords
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