The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage
- 1 December 2005
- journal article
- Published by Taylor & Francis Ltd in Molecular and Cellular Biology
- Vol. 25 (23), 10315-10328
- https://doi.org/10.1128/mcb.25.23.10315-10328.2005
Abstract
We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in vertebrate cells. To determine the precise role of CENP-50, we examined its role in centromere function by generating a loss-of-function mutant in the chicken DT40 cell line. The CENP-50 knockout was not lethal; however, the growth rate of cells with this mutation was slower than that of wild-type cells. We observed that the time for CENP-50-deficient cells to complete mitosis was longer than that for wild-type cells. Centromeric localization of CENP-50 was abolished in both CENP-H- and CENP-I-deficient cells. Coimmunoprecipitation experiments revealed that CENP-50 interacted with the CENP-H/CENP-I complex in chicken DT40 cells. We also observed severe mitotic defects in CENP-50-deficient cells with apparent premature sister chromatid separation when the mitotic checkpoint was activated, indicating that CENP-50 is required for recovery from spindle damage.Keywords
This publication has 46 references indexed in Scilit:
- A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tensionGenes & Development, 2004
- Dynamics of Centromere and Kinetochore ProteinsImplications for Checkpoint Signaling and SilencingCurrent Biology, 2004
- Dynamics of Centromere and Kinetochore Proteins: Implications for Checkpoint Signaling and SilencingCurrent Biology, 2004
- Assembly of kinetochores in vertebrate cellsExperimental Cell Research, 2004
- cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IPOncogene, 2004
- In vivo kinetics of Cajal body componentsThe Journal of cell biology, 2004
- Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cellsJournal of Cell Science, 2003
- The spindle checkpoint: structural insights into dynamic signallingNature Reviews Molecular Cell Biology, 2002
- CENP-I Is Essential for Centromere Function in Vertebrate CellsDevelopmental Cell, 2002
- Mammalian Centromeres: DNA Sequence, Protein Composition, and Role in Cell Cycle ProgressionExperimental Cell Research, 1999