Trends and advances in food analysis by real-time polymerase chain reaction
- 5 May 2016
- journal article
- review article
- Published by Springer Science and Business Media LLC in Journal of Food Science and Technology
- Vol. 53 (5), 2196-2209
- https://doi.org/10.1007/s13197-016-2205-0
Abstract
Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns. Real-time polymerase chain reaction (RT-PCR), a rapid and inexpensive quantitative method to detect the presence of targeted DNA-segments in samples, helps in determining both accidental and intentional adulterations of foods by biological contaminants. This review presents recent developments in theory, techniques, and applications of RT-PCR in food analyses, RT-PCR addresses the limitations of traditional food analyses in terms of sensitivity, range of analytes, multiplexing ability, cost, time, and point-of-care applications. A range of targets, including species of plants or animals which are used as food ingredients, food-borne bacteria or viruses, genetically modified organisms, and allergens, even in highly processed foods can be identified by RT-PCR, even at very low concentrations. Microfluidic RT-PCR eliminates the separate sample-processing step to create opportunities for point-of-care analyses. We also cover the challenges related to using RT-PCR for food analyses, such as the need to further improve sample handling.Keywords
Funding Information
- Brunei Research Council (BRC-10)
This publication has 70 references indexed in Scilit:
- Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: A tool for disclosure of olive oil adulterationFood Chemistry, 2013
- Eprobe Mediated Real-Time PCR Monitoring and Melting Curve AnalysisPLOS ONE, 2013
- Rapid Detection and Simultaneous Genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in Powdered Infant Formula Using Real-time PCR and High Resolution Melting (HRM) AnalysisPLOS ONE, 2013
- How to Deal with the Upcoming Challenges in GMO Detection in Food and FeedJournal of Biomedicine and Biotechnology, 2012
- Real-Time PCR-Based Mismatch Amplification Mutation Assay for Specific Detection of CS6-Expressing Allelic Variants of Enterotoxigenic Escherichia coli and Its Application in Assessing Diarrheal Cases and Asymptomatic ControlsJournal of Clinical Microbiology, 2012
- SYTO dyes and EvaGreen outperform SYBR Green in real-time PCRBMC Research Notes, 2011
- Simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products using real time PCR-melt curve analysisJournal of Food Science and Technology, 2011
- Application of mid-infrared spectroscopy with multivariate analysis and soft independent modeling of class analogies (SIMCA) for the detection of adulterants in minced beefMeat Science, 2010
- Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensorFood Control, 2010
- Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperatureNucleic Acids Research, 2007