Microarray‐based genomic profiling reveals novel genomic aberrations in follicular lymphoma which associate with patient survival and gene expression status
Open Access
- 30 September 2008
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
- Vol. 48 (1), 39-54
- https://doi.org/10.1002/gcc.20617
Abstract
Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array‐based intermediate‐high resolution genomic profiling in combination with Affymetrix™ gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence of genomic aberrations and the results were correlated to clinical data sets and mRNA expression levels. In 114 (89%) of the 128 analyzed cases, a total of 688 genomic aberrations (384 gains/amplifications and 304 losses) were detected. Frequent genomic aberrations were: −1p36 (18%), +2p15 (24%), −3q (14%), −6q (25%), +7p (19%), +7q (23%), +8q (14%), −9p (16%), −11q (15%), +12q (20%), −13q (11%), −17p (16%), +18p (18%), and +18q (28%). Critical segments of these imbalances were delineated to genomic fragments with a minimum size down to 0.2 Mb. By comparison of these with mRNA gene expression data, putative candidate genes were identified. Moreover, we found that deletions affecting the tumor suppressor gene CDKN2A/B on 9p21 were detected in nontransformed FL grade I–II. For this aberration as well as for −6q25 and −6q26, an association with inferior survival was observed.Keywords
This publication has 62 references indexed in Scilit:
- Gain of chromosome region 18q21 including the MALT1 gene is associated with the activated B-cell-like gene expression subtype and increased BCL2 gene dosage and protein expression in diffuse large B-cell lymphomaHaematologica, 2008
- Microrna expression distinguishes between germinal center B cell‐like and activated B cell‐like subtypes of diffuse large B cell lymphomaInternational Journal of Cancer, 2007
- Global variation in copy number in the human genomeNature, 2006
- The G12 family of heterotrimeric G proteins promotes breast cancer invasion and metastasisProceedings of the National Academy of Sciences, 2006
- Detection of large-scale variation in the human genomeNature Genetics, 2004
- Prediction of Survival in Diffuse Large-B-Cell Lymphoma Based on the Expression of Six GenesNew England Journal of Medicine, 2004
- Initial sequencing and analysis of the human genomeNature, 2001
- Comparative genomic hybridization detects genomic abnormalities in 80% of follicular lymphomasBritish Journal of Haematology, 1997
- Distinctive Chromosomal Abnormalities in Histologic Subtypes of Non-Hodgkin's LymphomaNew England Journal of Medicine, 1982
- Nonparametric Estimation from Incomplete ObservationsJournal of the American Statistical Association, 1958