Cationic stearylamine-containing biodegradable microparticles for DNA delivery
- 1 February 2004
- journal article
- research article
- Published by Informa UK Limited in Journal of Microencapsulation
- Vol. 21 (1), 25-36
- https://doi.org/10.1080/02652040410001653777
Abstract
Technical aspects for preparing a new type of cationic stearylamine (SA)-containing microparticle as a potential drug delivery system for negatively charged therapeutics were investigated. Cationic biodegradable microparticles based on poly(lactide) and poly(lactide-co-glycolide) were prepared upon incorporation of SA either by solvent evaporation or by spray-drying. Water-insoluble SA offers the advantage over other water-soluble cationic compounds that it can be dissolved directly in the organic solution together with the polymers. This facilitated the subsequent preparation of the microparticle formulations. Particle size was controlled by the respective process parameters, resulting in either large polymer aggregates within the range 50–100 µm or small spherical microparticles within the range 1–10 µm. The incorporation of SA into the formulations also improved particle characteristics in terms of re-dispersibility, reduced sticking, and particle size uniformity. Both circular plasmid DNA (5 kbp) and linear salmon DNA (0.5 kbp) were efficiently adsorbed to the cationic SA microparticle surfaces. Preliminary tests on the release of DNA from spray-dried SA microparticles showed an immediate burst release, which was followed by a delayed second release phase for more than 4 weeks. The cationic SA microparticles might provide a potential drug-delivery system to improve the efficacy for protein and DNA-type therapeutics.Keywords
This publication has 14 references indexed in Scilit:
- Evaluation of cationic solid lipid microparticles as synthetic carriers for the targeted delivery of macromolecules to phagocytic antigen-presenting cellsBiomaterials, 2002
- Uptake of poly(D,L‐lactic‐co‐glycolic acid) microspheres by antigen‐presenting cells in vivoJournal of Biomedical Materials Research, 2002
- Microparticle-mediated Transfection of Non-phagocytic Cells In VitroJournal of Drug Targeting, 2002
- Transplantation of brain cells assembled around a programmable synthetic microenvironmentNature Biotechnology, 2001
- Hydrophilic poly(dl-lactide-co-glycolide) microspheres for the delivery of DNA to human-derived macrophages and dendritic cellsJournal of Controlled Release, 2001
- In VivoUptake of Chitosan Microparticles by Murine Peyer's Patches: Visualization Studies using Confocal Laser Scanning Microscopy and ImmunohistochemistryJournal of Drug Targeting, 2001
- Effects of Physicochemical Characteristics of Poly(2-(dimethylamino)ethyl methacrylate)-Based Polyplexes on Cellular Association and InternalizationJournal of Drug Targeting, 2000
- Oral particulate delivery: status and future trendsAdvanced Drug Delivery Reviews, 1998
- Human Gene TherapyCritical Reviews in Biotechnology, 1997
- Tetanus toxoid and synthetic malaria antigen containing poly(lactide)/poly(lactide-co-glycolide) microspheres: importance of polymer degradation and antigen release for immune responseJournal of Controlled Release, 1996