Key developmental regulators change during hyperoxia‐induced injury and recovery in adult mouse lung
- 13 December 2006
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 100 (6), 1415-1429
- https://doi.org/10.1002/jcb.21142
Abstract
Developmentally important genes have recently been linked to tissue regeneration and epithelial cell repair in neonatal and adult animals in several organs, including liver, skin, prostate, and musculature. We hypothesized that developmentally important genes play roles in lung injury repair in adult mice. Although there is considerable information known about these processes, the specific molecular pathways that mediate injury and regulate tissue repair are not fully elucidated. Using a hyperoxic injury model to study these mechanisms of lung injury and tissue repair, we selected the following genes based upon their known or putative roles in lung development and organogenesis: TTF-1, FGF9, FGF10, BMP4, PDGF-A, VEGF, Ptc, Shh, Sca-1, BCRP, CD45, and Cyclin-D2. Our findings demonstrate that several developmentally important genes (Sca-1, Shh, PDGF-A, VEGF, BCRP, CD45, BMP4, and Cyclin-D2) change during hyperoxic injury and normoxic recovery in mice, suggesting that adult lung may reactivate key developmental regulatory pathways for tissue repair. The mRNA for one gene (TTF-1), unchanged during hyperoxia, was upregulated late in recovery phase. These novel findings provide the basis for testing the efficacy of post-injury lung repair in animals genetically modified to inactivate or express individual molecules. J. Cell. Biochem. 100: 1415–1429, 2007.Keywords
This publication has 54 references indexed in Scilit:
- Effect of nitric oxide on fibroblast growth factor-10 and bone morphogenetic protein 4 expressions in the branching morphogenesis of fetal rat lung explantsJournal of Pediatric Surgery, 2005
- The fibroblast growth factor binding protein is a novel interaction partner of FGF-7, FGF-10 and FGF-22 and regulates FGF activity: implications for epithelial repairOncogene, 2005
- Hyperoxia-induced emphysematous changes in subacute phase of endotoxin-induced lung injury in ratsAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2004
- FGF signaling is required for pulmonary homeostasis following hyperoxiaAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2004
- Embryonic myogenesis pathways in muscle regenerationDevelopmental Dynamics, 2004
- Enhanced binding of Sp1/Sp3 transcription factors mediates the hyperoxia-induced increased expression of the lung type I cell geneT1?Journal of Cellular Biochemistry, 2003
- Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancerNature, 2003
- Expression of the developmental Sonic hedgehog (Shh) signalling pathway is up‐regulated in chronic lung fibrosis and the Shh receptor patched 1 is present in circulating T lymphocytesThe Journal of Pathology, 2003
- Haploinsufficient Phenotypes inBmp4Heterozygous Null Mice and Modification by Mutations inGli3andAlx4Developmental Biology, 1997
- Intratracheal instillation of keratinocyte growth factor decreases hyperoxia-induced mortality in rats.JCI Insight, 1995