MS‐based analytical methodologies to characterize genetically modified crops
- 23 December 2010
- journal article
- review article
- Published by Wiley in Mass Spectrometry Reviews
- Vol. 30 (3), 396-416
- https://doi.org/10.1002/mas.20286
Abstract
El pdf del artículo es la versión pre-print.The development of genetically modified crops has had a great impact on the agriculture and food industries. However, the development of any genetically modified organism (GMO) requires the application of analytical procedures to confirm the equivalence of the GMO compared to its isogenic non-transgenic counterpart. Moreover, the use of GMOs in foods and agriculture faces numerous criticisms from consumers and ecological organizations that have led some countries to regulate their production, growth, and commercialization. These regulations have brought about the need of new and more powerful analytical methods to face the complexity of this topic. In this regard, MS-based technologies are increasingly used for GMOs analysis to provide very useful information on GMO composition (e.g., metabolites, proteins). This review focuses on the MS-based analytical methodologies used to characterize genetically modified crops (also called transgenic crops). First, an overview on genetically modified crops development is provided, together with the main difficulties of their analysis. Next, the different MS-based analytical approaches applied to characterize GM crops are critically discussed, and include “-omics” approaches and target-based approaches. These methodologies allow the study of intended and unintended effects that result from the genetic transformation. This information is considered to be essential to corroborate (or not) the equivalence of the GM crop with its isogenic non-transgenic counterpart.Peer RevieweKeywords
Funding Information
- Ministerio de Ciencia e Innovación (AGL2008-05108-C03-01)
- Programa CONSOLIDER, Ministerio de Educacion y Ciencia (CSD2007-00063)
This publication has 104 references indexed in Scilit:
- Advances in Nutrigenomics research: Novel and future analytical approaches to investigate the biological activity of natural compounds and food functionsJournal of Pharmaceutical and Biomedical Analysis, 2010
- Metabolomics of transgenic maize combining Fourier transform-ion cyclotron resonance-mass spectrometry, capillary electrophoresis-mass spectrometry and pressurized liquid extractionJournal of Chromatography A, 2009
- Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection for Single-Cell MetabolomicsAnalytical Chemistry, 2009
- New trends in bioanalytical tools for the detection of genetically modified organisms: an updateAnalytical and Bioanalytical Chemistry, 2008
- Use of supercritical fluid extraction and gas chromatography–mass spectrometry to obtain amino acid profiles from several genetically modified varieties of maize and soybeanJournal of Chromatography A, 2008
- An HPLC-ICP-MS technique for determination of cadmium–phytochelatins in genetically modified Arabidopsis thalianaJournal of Chromatography B, 2008
- Over-expression of calcium-dependent protein kinase 13 and calreticulin interacting protein 1 confers cold tolerance on rice plantsMolecular Genetics and Genomics, 2007
- FTICR mass spectrometry for qualitative and quantitative bioanalysesCurrent Opinion in Biotechnology, 2004
- Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: The case of a virus‐resistant tomatoProteomics, 2004
- Fourier transform ion cyclotron resonance mass spectrometry: A primerMass Spectrometry Reviews, 1998