GermSAGE: a comprehensive SAGE database for transcript discovery on male germ cell development
Open Access
- 2 October 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 37 (Database), D891-D897
- https://doi.org/10.1093/nar/gkn644
Abstract
GermSAGE is a comprehensive web-based database generated by Serial Analysis of Gene Expression (SAGE) representing major stages in mouse male germ cell development, with 150 000 sequence tags in each SAGE library. A total of 452 095 tags derived from type A spermatogonia (Spga), pachytene spermatocytes (Spcy) and round spermatids (Sptd) were included. GermSAGE provides web-based tools for browsing, comparing and searching male germ cell transcriptome data at different stages with customizable searching parameters. The data can be visualized in a tabulated format or further analyzed by aligning with various annotations available in the UCSC genome browser. This flexible platform will be useful for gaining better understanding of the genetic networks that regulate spermatogonial cell renewal and differentiation, and will allow novel gene discovery. GermSAGE is freely available at http://germsage.nichd.nih.gov/Keywords
This publication has 13 references indexed in Scilit:
- UCSC genome browser tutorialGenomics, 2008
- The UCSC Genome Browser Database: 2008 updateNucleic Acids Research, 2007
- The Genome Browser at UCSC for Locating Genes, and Much More!Molecular Biotechnology, 2007
- The protamine family of sperm nuclear proteinsGenome Biology, 2007
- Application of transcriptional and biological network analyses in mouse germ-cell transcriptomesGenomics, 2006
- The complexity of antisense transcription revealed by the study of developing male germ cellsGenomics, 2006
- Analysis of mouse germ-cell transcriptome at different stages of spermatogenesis by SAGE: Biological significanceGenomics, 2004
- Database resources of the National Center for Biotechnology Information: updateNucleic Acids Research, 2004
- SAGEmap: A Public Gene Expression ResourceGenome Research, 2000
- Serial Analysis of Gene ExpressionScience, 1995