High frequency of methylenetetrahydrofolate reductase 677TT genotype in Hungarian HELLP syndrome patients determined by quantitative real-time PCR
Open Access
- 30 November 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC in Journal of Human Hypertension
- Vol. 21 (2), 154-158
- https://doi.org/10.1038/sj.jhh.1002122
Abstract
Our aim was to determine the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in hypertensive disorders during pregnancy. We conducted our experiments on isolated DNA samples of 73 healthy pregnant, 101 severe pre-eclamptic and 63 HELLP syndrome women in this study. The MTHFR C677T polymorphism was determined by quantitative real-time PCR method. A significantly higher number of the TT genotype (25.4%) was found in the HELLP syndrome group compared to the healthy (8.2%) and severe pre-eclamptics group (8.9%) (P=0.03). The frequency of the mutant T allele was found to be 45.2% of HELLP syndrome, whereas it was 32.2% of the healthy pregnant (P=0.03) and 30.2% (P=0.008) of the severe pre-eclamptic patients. In the HELLP group a high frequency of eclampsia was observed (12.6%) and among them 75% had the MTHFR C677T mutation.This publication has 24 references indexed in Scilit:
- The DNA isolation method has effect on allele drop-out and on the results of fluorescent PCR and DNA fragment analysisClinica Chimica Acta; International Journal of Clinical Chemistry, 2005
- Methylenetetrahydrofolate reductase C677T polymorphism and Factor V Leiden variant in Mexican women with preeclampsia/eclampsiaBlood Cells, Molecules, and Diseases, 2005
- Searching for preeclampsia genes: the current positionEuropean Journal of Obstetrics & Gynecology and Reproductive Biology, 2002
- Hyperhomocysteinaemia: a risk factor for preeclampsia?European Journal of Obstetrics & Gynecology and Reproductive Biology, 2001
- Prothrombin 20210 G→A, MTHFR C677T mutations in women with venous thromboembolism associated with pregnancyBJOG: An International Journal of Obstetrics and Gynaecology, 2000
- Factor V Leiden and Thermolabile Methylenetetrahydrofolate Reductase Gene Variants in an East Anglian Preeclampsia CohortHypertension, 1999
- Detection of factor V Leiden mutation in severe pre‐eclamptic Hungarian womenClinical Genetics, 1998
- Dyslipidemia, Iron, and Oxidative Stress in Preeclampsia: Assessment of Maternal and Feto-Placental InteractionsSeminars in Reproductive Medicine, 1998
- Methylenetetrahydrofolate reductase polymorphism and pre-eclampsia.Journal of Medical Genetics, 1997
- Heterogeneous causes constituting the single syndrome of preeclampsia: A hypothesis and its implicationsAmerican Journal of Obstetrics and Gynecology, 1996