DNA Origami with Complex Curvatures in Three-Dimensional Space
Top Cited Papers
- 15 April 2011
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 332 (6027), 342-346
- https://doi.org/10.1126/science.1202998
Abstract
We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature—such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask—were assembled.Keywords
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