1,25-Dihydroxyvitamin D in Biological Fluids: A Simplified and Sensitive Assay
- 10 September 1976
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 193 (4257), 1021-1023
- https://doi.org/10.1126/science.1085035
Abstract
A competitive binding assay for 1,25-dihydroxyvitamin D [1,25-(OH2D] in plasma has been developed in which intestinal cytosol preparations from rachitic chicks are used as the binding protein. A new method of extraction and two new chromatographic procedures are used for this assay. The method is sensitive to as little as 10 picograms of 1,25-(OH)2D, and triplicate assays can be done on 5 milliliters of plasma. This assay shows that in the plasma of normal adult subjects there is a 1,25-(OH)2D concentration of 29 +/- 2 picograms per milliliter, while none can be detected in the plasma of nephrectomized subjects and end-stage renal failure patients.This publication has 21 references indexed in Scilit:
- Filter assay for 1α,25-dihydroxyvitamin D3. Utilization of the hormone's target tissue chromatin receptorBiochemistry, 1974
- The role of vitamin D metabolites in bone resorptionCalcified Tissue International, 1973
- Intestinal Calcium Transport: Stimulation by Low Phosphorus DietsScience, 1973
- The control of 25-hydroxyvitamin D metabolism by inorganic phosphorusArchives of Biochemistry and Biophysics, 1973
- Bone mineral mobilization activity of 1,25-dihydroxycholecalciferol, a metabolite of vitamin DArchives of Biochemistry and Biophysics, 1971
- Synthesis of [26,27-3H]-25-hydroxycholecalciferolAnalytical Biochemistry, 1971
- Biological activity 1,25-dihydroxycholecalciferolBiochemistry, 1971
- Vitamin D Metabolism: The Role of Kidney TissueScience, 1971
- Vitamin D: A Cholecalciferol Metabolite Highly Active in Promoting Intestinal Calcium TransportScience, 1971
- Unique Biosynthesis by Kidney of a Biologically Active Vitamin D MetaboliteNature, 1970