The sequences of Arabidopsis GA‐INSENSITIVE RNA constitute the motifs that are necessary and sufficient for RNA long‐distance trafficking
- 9 September 2009
- journal article
- Published by Wiley in The Plant Journal
- Vol. 59 (6), 921-929
- https://doi.org/10.1111/j.1365-313x.2009.03918.x
Abstract
P>In higher plants a number of physiological processes are regulated by systemic RNA signaling molecules. This phloem-mediated remote-control system provides specific and efficient regulation to fine-tune many plant developmental programs. However, the molecular mechanism underlying long-distance movement of RNA remains to be elucidated. To this end, we examined the long-distance movement of GA-insensitive (GAI) RNA by Arabidopsis inflorescence grafting and RT-PCR analysis. Our results demonstrated that long-distance movement of RNA only occurred in specific transcripts. In addition, the sequences of GAI RNA are necessary and sufficient to target GREEN FLUORESCENT PROTEIN (GFP) RNA for long-distance movement, which indicates that the trafficking of GAI RNA is mediated by specific RNA motifs. Further analyses revealed that the motifs at coding sequences and 3' untranslated regions of GAI RNA play important roles during RNA movement. In addition, the structure of the RNA rather than its specific sequence may also be important in GAI RNA trafficking. However, the secondary structure of GAI RNA is not the only factor to target RNA for long-distance movement, because recovery of the secondary structure of movement-defective GAI RNA only partially rescued RNA movement. Taken together, our results show that long-distance movement of non-cell autonomous RNA operates by specific RNA mobile elementsKeywords
This publication has 43 references indexed in Scilit:
- A Polypyrimidine Tract Binding Protein, Pumpkin RBP50, Forms the Basis of a Phloem-Mobile Ribonucleoprotein ComplexPlant Cell, 2009
- Regulatory Network of MicroRNA399 andPHO2by Systemic SignalingPlant Physiology, 2008
- A Genomic Map of Viroid RNA Motifs Critical for Replication and Systemic TraffickingPlant Cell, 2008
- MicroRNA399 is a long‐distance signal for the regulation of plant phosphate homeostasisThe Plant Journal, 2007
- Tertiary structure and function of an RNA motif required for plant vascular entry to initiate systemic traffickingThe EMBO Journal, 2007
- Dynamics of a Mobile RNA of Potato Involved in a Long-Distance Signaling PathwayPlant Cell, 2006
- A novel cell-to-cell trafficking assay indicates that the KNOX homeodomain is necessary and sufficient for intercellular protein and mRNA traffickingGenes & Development, 2005
- Direct Role of a Viroid RNA Motif in Mediating Directional RNA Trafficking across a Specific Cellular Boundary[W]Plant Cell, 2004
- RNP Localization and Transport in YeastAnnual Review of Cell and Developmental Biology, 2001
- ASH1 mRNA localization in yeast involves multiple secondary structural elementsand Ash1 protein translationCurrent Biology, 1999