α-Enolase Expressed on the Surfaces of Monocytes and Macrophages Induces Robust Synovial Inflammation in Rheumatoid Arthritis
Open Access
- 1 July 2012
- journal article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 189 (1), 365-372
- https://doi.org/10.4049/jimmunol.1102073
Abstract
α-Enolase (ENO1) is a multifunctional glycolytic enzyme expressed abundantly in the cytosol. It has been implicated in autoimmune and inflammatory diseases. Serum Abs against ENO1 were reported in rheumatoid arthritis (RA). Cell-surface expression of ENO1 has been found to be increased rapidly in response to inflammatory stimuli, but its expression and function has not been reported in RA. In this study, we show that cell-surface expression of ENO1 is increased on monocytes and macrophages isolated from RA patients but not on those from osteoarthritis patients, and Ab against ENO1 can stimulate these cells to produce higher amounts of proinflammatory mediators, such as TNF-α, IL-1 α/β, IFN-γ, and PGE2 via p38 MAPK and NF-κB pathway. The frequency of ENO1-positive cells in synovial fluid mononuclear cells was higher than PBMCs. ENO1-positive cells were also found in the inflamed synovium from RA patients and arthritic ankle tissues of mice with collagen-induced arthritis. Taken together, these findings suggest that Abs against ENO1 present in RA sera may stimulate monocytes and macrophages expressing cell-surface ENO1 and contribute to production of proinflammatory mediators during the effector phase of synovial inflammation.Keywords
This publication has 31 references indexed in Scilit:
- Review: Immune cells and mediators of inflammatory arthritisAutoimmunity, 2008
- Macrophages and their products in rheumatoid arthritisCurrent Opinion in Rheumatology, 2007
- Update on cytokines in rheumatoid arthritisCurrent Opinion in Rheumatology, 2007
- Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against α‐EnolaseAmerican Journal of Hematology, 2003
- Rheumatoid arthritisThe Lancet, 2001
- ROLE OF CYTOKINES IN RHEUMATOID ARTHRITISAnnual Review of Immunology, 1996
- The Role of an Enolase‐Related Molecule in Plasminogen Binding to CellsJBIC Journal of Biological Inorganic Chemistry, 1995
- Role of cell-surface lysines in plasminogen binding to cells: identification of .alpha.-enolase as a candidate plasminogen receptorBiochemistry, 1991
- Isolation and partial characterization of a 48-kDa protein which is induced in normal lymphocytes upon mitogenic stimulationBiochemical and Biophysical Research Communications, 1986
- Predominantly T-Cell Infiltrate in Rheumatoid Synovial MembranesThe New England Journal of Medicine, 1975