Behavioural and neural deficits induced by rotenone in the pond snailLymnaea stagnalis. A possible model for Parkinson's disease in an invertebrate
- 1 April 2007
- journal article
- Published by Wiley in European Journal of Neuroscience
- Vol. 25 (7), 2123-2130
- https://doi.org/10.1111/j.1460-9568.2007.05467.x
Abstract
Parkinson's disease is a neurodegenerative disorder, related to the loss of dopamine (DA)-containing neurons in the substantia nigra. In experimental animals, both vertebrates and invertebrates, rotenone, a commercially available organic pesticide, induces symptoms of Parkinson's disease. We found that that rotenone is toxic to the pond snail Lymnaea stagnalis (4-day LC50 0.8 microM). Rotenone, at concentrations from 0.1 to 5 microM, caused progressive and irreversible behavioural deficits in both acute and chronic exposure. Chronic exposure to 0.5 microM rotenone led to a progressive decrease in spontaneous locomotion and in feeding, reaching almost 100% inhibition of both behaviours by the 7th day of rotenone treatment. In the central nervous system preparation made on the 7th day of treatment the postsynaptic potentials evoked by the identified dopaminergic RPeD1 neuron disappeared whereas the synaptic inputs received by the RPeD1 from a peptidergic neuron (VD4) were still functional. Immunostaining revealed that the tyrosine hydroxylase immunoreactivity decreased below the detectable level in both the RPeD1 cell body and its axonal processes. Finally, HPLC assay showed a significant (25%) decrease in DA level in the CNS by the 7th day of rotenone treatment. We conclude that, as in vertebrates, rotenone disrupts feeding and locomotion of the model mollusc Lymnaea stagnalis. One possible target of rotenone is the dopaminergic neurons in the CNS. We therefore suggest that Lymnaea stagnalis is a suitable invertebrate model for the study of Parkinson's disease, allowing direct analysis of the response of dopaminergic systems to rotenone at behavioural, cellular and neuronal levels.Keywords
This publication has 36 references indexed in Scilit:
- An identified central pattern‐generating neuron co‐ordinates sensory‐motor components of respiratory behavior in LymnaeaEuropean Journal of Neuroscience, 2006
- Rotenone and CCCP inhibit tyrosine hydroxylation in rat striatal tissue slicesToxicology, 2005
- Increased sensitivity of striatal dopamine release to H2O2 upon chronic rotenone treatmentFree Radical Biology & Medicine, 2005
- Neuroanatomical, immunocytochemical, and physiological studies of the pharyngeal retractor muscle and its putative regulatory neurons playing a role in withdrawal and feeding in the snail, Helix pomatiaCell and tissue research, 2005
- Parkinson's Disease: Mechanisms and ModelsNeuron, 2003
- Dopamine‐immunoreactive neurones in the central nervous system of the pond snail Lymnaea stagnalisJournal of Comparative Neurology, 1991
- In Vitro Reconstruction of the Respiratory Central Pattern Generator of the Mollusk LymnaeaScience, 1990
- Behavioural and biochemical changes in the feeding system of Lymnaea induced by the dopamine and serotonin neurotoxins 6-hydroxydopamine and 5,6-dihydroxytryptaminePhilosophical Transactions B, 1990
- Effect of putative neuromodulators on rhythmic buccal motor output inLymnaea stagnalisJournal of Neurobiology, 1989
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981