Changing medium and passaging cell lines
- 13 September 2007
- journal article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 2 (9), 2276-2284
- https://doi.org/10.1038/nprot.2007.319
Abstract
Cell lines are widely used in biomedical research. This protocol describes the methods used routinely to change the medium and passage the cells. Medium changes keep the cells healthy by providing fresh nutrients, while cell passage or splitting is required to maintain cells in exponential growth. Despite the simplicity of the methods used, each cell line has idiosyncracies. Whether working with one or several cell lines, there is no substitute for knowledge of their needs, including the range of phenotypes and growth patterns under different physical and nutrient conditions. Given the necessary care and attention, most cell lines are easy to maintain and grow.Keywords
This publication has 7 references indexed in Scilit:
- Guidance on Good Cell Culture PracticeAlternatives to Laboratory Animals, 2005
- TrueBritish Journal of Cancer, 2000
- Hepatitis B transmission from contaminated cryopreservation tankThe Lancet, 1995
- Needle-Stick Transmission of Human Colonic AdenocarcinomaThe New England Journal of Medicine, 1986
- Infection of laboratory workers with hantavirus acquired from immunocytomas propagated in laboratory ratsJournal of Infection, 1986
- Usage of vertebrate, invertebrate and plant cell, tissue and organ culture terminologyIn Vitro Cellular & Developmental Biology - Plant, 1984
- Encephalomyelitis Due to Infection withHerpesvirus simiae(Herpes B Virus)The New England Journal of Medicine, 1959