Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron
Open Access
- 1 March 2005
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 55 (3), 371-374
- https://doi.org/10.1093/jac/dkh534
Abstract
Objectives: To characterize class 1 and class 2 integrons among non-typhoid Salmonella enterica serovars in Japan, and also to monitor the spread of the multidrug-resistant S. enterica serovar Typhimurium DT104. Methods: A total of 105 Salmonella isolates were included in this study. The broth microdilution method was used to determine the MIC values of a range of antibiotics for these isolates. PCR and DNA sequencing were used for screening and characterization of class 1 and class 2 integrons. Results: PCR sequencing analysis revealed the presence of seven profiles of class 1 integrons in addition to a new type of class 2 integron. The identified gene cassettes within class 1 integrons were as follows; aadA1, aadA2 and aadA5, which confer resistance to streptomycin and spectinomycin; aadB, which confers resistance to gentamicin, kanamycin and tobramycin; dfrA1 and dfrA17, which confer resistance to trimethoprim; blaPSE-1, which confers resistance to ampicillin; catB3, which confers resistance to chloramphenicol; and sat1, which confers resistance to streptothricin. Two strains of the multidrug-resistant S. Typhimurium DT104 were characterized in this study. DNA sequencing of class 2 integrons identified one with an unusual array of gene cassettes, sat, sat1 and aadA1. Conclusions: In this study, we characterized the antibiotic resistance gene cassettes within class 1 integrons in different isolates of non-typhoid Salmonella serovars, and we also identified a new type of class 2 integron.Keywords
This publication has 7 references indexed in Scilit:
- Erythromycin Esterase Gene ere (A) Is Located in a Functional Gene Cassette in an Unusual Class 2 IntegronAntimicrobial Agents and Chemotherapy, 2003
- Antimicrobial Resistance and Genetic Diversity of Shigella sonnei Isolates from Western Ireland, an Area of Low Incidence of InfectionJournal of Clinical Microbiology, 2003
- IntI2 Integron Integrase in Tn7Journal of Bacteriology, 2002
- Bacterial resistance evolution by recruitment of super‐integron gene cassettesMolecular Microbiology, 2002
- Incidence of Class 1 and 2 Integrases in Clinical and Commensal Bacteria from Livestock, Companion Animals, and ExoticsAntimicrobial Agents and Chemotherapy, 2001
- Emergence of Multidrug-ResistantSalmonella entericaSerotypeTyphimurium DT104 Infections in the United StatesNew England Journal of Medicine, 1998
- Epidemic in cattle and humans of Salmonella typhimurium DT 104 with chromosomally integrated multiple drug resistancePublished by Wiley ,1994