Follow up of infection of chacma baboons with inoculum containing a and non-a genotypes of hepatitis B virus
Open Access
- 1 January 2003
- journal article
- Published by Baishideng Publishing Group Inc. in World Journal of Gastroenterology
- Vol. 9 (4), 731-735
- https://doi.org/10.3748/wjg.v9.i4.731
Abstract
AIM: To determine whether one genotype (A or non-A genotypes of HBV) predominated over the other during the course of HBV infection. METHODS: Four baboons were inoculated with HBV. DNA was extracted from serum obtained at monthly intervals post-inoculation for 52 weeks and HBV DNA was amplified using primers specific for the core region containing an insert characteristic of genotype A (nt 2354-2359, numbering from the EcoRI site). The amplicons were cloned into PCR-ScriptTM and a minimum of 15 clones per time point were sequenced in both directions. RESULTS: Both genotypes persisted for the entire follow-up period of 52 weeks. Genotype non-A predominated in two baboons and genotype A in one baboon. Neither genotype predominated in the fourth baboon, as shown at a 5% level of testing. CONCLUSION: No conclusions concerning the dominance of either genotype or the natural progression or replication rates of HBV could be drawn because the pattern of the genotypes found may have been caused by sampling fluctuations at the time of DNA extraction and cloning as a result of the very low viral loads in the baboon sera.Keywords
This publication has 25 references indexed in Scilit:
- Characterization of two hepatitis B virus populations isolated from a hepatitis B surface antigen-negative patientHepatology, 2002
- Hepatitis C After Liver TransplantationSeminars in Liver Disease, 2000
- Does hepatitis B virus (HBV) genotype influence the clinical outcome of HBV infection?Journal of Viral Hepatitis, 1999
- Nucleic acid sequence analysis of the precore region of hepatitis B virus from sera of southern African black adult carriers of the virusHepatology, 1997
- Hepatitis B surface antigen particles with all four subtypic determinants: Point mutations of hepatitis B virus DNA inducing phenotypic changes or double infection with viruses of different subtypesMolecular Immunology, 1990
- Avoiding false positives with PCRNature, 1989
- Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen SubtypesJournal of General Virology, 1988
- Use of monoclonal antibodies to determine if HBsAg of mixed subtype is one particle or twoJournal of Virological Methods, 1986
- Distribution of HBsAg Subtypes in the WorldVox Sanguinis, 1983
- South-to-North Gradient in Distribution of the r Determinant of Hepatitis B Surface Antigen in JapanThe Journal of Infectious Diseases, 1975