Derivation and Cloning of a Novel Rhesus Embryonic Stem Cell Line Stably Expressing Tau-Green Fluorescent Protein
Open Access
- 20 March 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in The International Journal of Cell Cloning
- Vol. 26 (6), 1444-1453
- https://doi.org/10.1634/stemcells.2007-0953
Abstract
Embryonic stem cells (ESC) have the ability of indefinite self-renewal and multilineage differentiation, and they carry great potential in cell-based therapies. The rhesus macaque is the most relevant preclinical model for assessing the benefit, safety, and efficacy of ESC-based transplantations in the treatment of neurodegenerative diseases. In the case of neural cell grafting, tracing both the neurons and their axonal projections in vivo is essential for studying the integration of the grafted cells in the host brain. Tau-Green fluorescent protein (tau-GFP) is a powerful viable lineage tracer, allowing visualization of cell bodies, dendrites, and axons in exquisite detail. Here, we report the first rhesus monkey ESC line that ubiquitously and stably expresses tau-GFP. First, we derived a new line of rhesus monkey ESC (LYON-ES1) that show marker expression and cell cycle characteristics typical of primate ESCs. LYON-ES1 cells are pluripotent, giving rise to derivatives of the three germ layers in vitro and in vivo through teratoma formation. They retain all their undifferentiated characteristics and a normal karyotype after prolonged culture. Using lentiviral infection, we then generated a monkey ESC line stably expressing tau-GFP that retains all the characteristics of the parental wild-type line and is clonogenic. We show that neural precursors derived from the tau-GFP ESC line are multipotent and that their fate can be precisely mapped in vivo after grafting in the adult rat brain. Disclosure of potential conflicts of interest is found at the end of this article.Keywords
This publication has 39 references indexed in Scilit:
- Pedigreed Primate Embryonic Stem Cells Express Homogeneous Familial Gene ProfilesThe International Journal of Cell Cloning, 2007
- Clonal Analysis of Human Embryonic Stem Cell Differentiation into TeratomasThe International Journal of Cell Cloning, 2007
- Bone Morphogenetic Protein-4 Enhances Cardiomyocyte Differentiation of Cynomolgus Monkey ESCs in Knockout Serum Replacement MediumThe International Journal of Cell Cloning, 2006
- Transplanted dopamine neurons derived from primate ES cells preferentially innervate DARPP‐32 striatal progenitors within the graftEuropean Journal of Neuroscience, 2006
- Isolation and Characterization of Novel Rhesus Monkey Embryonic Stem Cell LinesThe International Journal of Cell Cloning, 2006
- Self‐renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phaseJournal of Cellular Physiology, 2006
- Fluorescence-Activated Single Cell Sorting of Human Embryonic Stem CellsCloning and Stem Cells, 2006
- Cell Cycle Features of Primate Embryonic Stem CellsThe International Journal of Cell Cloning, 2005
- Transplantation of neural cells derived from retinoic acid-treated cynomolgus monkey embryonic stem cells successfully improved motor function of hemiplegic mice with experimental brain injuryNeurobiology of Disease, 2005
- Differentiation of Monkey Embryonic Stem Cells into Neural Lineages1Biology of Reproduction, 2003