CD161 (NKR-P1A) Costimulation of CD1d-dependent Activation of Human T Cells Expressing Invariant Vα24JαQ T Cell Receptor α Chains
Open Access
- 7 September 1998
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 188 (5), 867-876
- https://doi.org/10.1084/jem.188.5.867
Abstract
A population of human T cells expressing an invariant Vα24JαQ T cell antigen receptor (TCR) α chain and high levels of CD161 (NKR-P1A) appears to play an immunoregulatory role through production of both T helper (Th) type 1 and Th2 cytokines. Unlike other CD161+ T cells, the major histocompatibility complex–like nonpolymorphic CD1d molecule is the target for the TCR expressed by these T cells (Vα24invt T cells) and by the homologous murine NK1 (NKR-P1C)+ T cell population. In this report, CD161 was shown to act as a specific costimulatory molecule for TCR-mediated proliferation and cytokine secretion by Vα24invt T cells. However, in contrast to results in the mouse, ligation of CD161 in the absence of TCR stimulation did not result in Vα24invt T cell activation, and costimulation through CD161 did not cause polarization of the cytokine secretion pattern. CD161 monoclonal antibodies specifically inhibited Vα24invt T cell proliferation and cytokine secretion in response to CD1d+ target cells, demonstrating a physiological accessory molecule function for CD161. However, CD1d-restricted target cell lysis by activated Vα24invt T cells, which involved a granule-mediated exocytotic mechanism, was CD161-independent. In further contrast to the mouse, the signaling pathway involved in Vα24invt T cell costimulation through CD161 did not appear to involve stable association with tyrosine kinase p56Lck. These results demonstrate a role for CD161 as a novel costimulatory molecule for TCR-mediated recognition of CD1d by human Vα24invt T cells.Keywords
This publication has 56 references indexed in Scilit:
- MOUSE CD1-SPECIFIC NK1 T CELLS: Development, Specificity, and FunctionAnnual Review of Immunology, 1997
- Early quantitative and functional deficiency of NK1+‐like thymocytes in the NOD mouseEuropean Journal of Immunology, 1996
- Beta 2-microglobulin-dependent T cells are dispensable for allergen-induced T helper 2 responses.The Journal of Experimental Medicine, 1996
- Human T cells expressing an invariant Va24-JotQ TCRα are CD4- and heterogeneous with respect to TCRβ expressionHuman Immunology, 1996
- Role of NK1.1 + T Cells in a T H 2 Response and in Immunoglobulin E ProductionScience, 1995
- CD1 Recognition by Mouse NK1 + T LymphocytesScience, 1995
- p40, a novel surface molecule involved in the regulation of the non‐major histocompatibility complex‐restricted cytolytic activity in humansEuropean Journal of Immunology, 1995
- A Subset of CD4 + Thymocytes Selected by MHC Class I MoleculesScience, 1994
- CDlb restricts the response of human CD4−8−T lymphocytes to a microbial antigenNature, 1992
- Recognition of cluster of differentiation 1 antigens by human CD4−CD8>− cytolytic T lymphocyteNature, 1989