Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution
Top Cited Papers
Open Access
- 12 May 2010
- journal article
- Published by Optica Publishing Group in Optics Express
- Vol. 18 (11), 11181-11191
- https://doi.org/10.1364/oe.18.011181
Abstract
We demonstrate lensfree holographic microscopy on a chip to achieve ~0.6 µm spatial resolution corresponding to a numerical aperture of ~0.5 over a large field-of-view of ~24 mm2. By using partially coherent illumination from a large aperture (~50 µm), we acquire lower resolution lensfree in-line holograms of the objects with unit fringe magnification. For each lensfree hologram, the pixel size at the sensor chip limits the spatial resolution of the reconstructed image. To circumvent this limitation, we implement a sub-pixel shifting based super-resolution algorithm to effectively recover much higher resolution digital holograms of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area, which is also equivalent to the imaging field-of-view (24 mm2) due to unit magnification. We demonstrate the success of this pixel super-resolution approach by imaging patterned transparent substrates, blood smear samples, as well as Caenoharbditis Elegans.Keywords
This publication has 24 references indexed in Scilit:
- On-chip differential interference contrast microscopy using lensless digital holographyOptics Express, 2010
- Imaging Intracellular Fluorescent Proteins at Nanometer ResolutionScience, 2006
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)Nature Methods, 2006
- Multiaperture imagingApplied Optics, 2006
- Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolutionProceedings of the National Academy of Sciences of the United States of America, 2005
- Super-resolution image reconstruction: a technical overviewIEEE Signal Processing Magazine, 2003
- Joint MAP registration and high-resolution image estimation using a sequence of undersampled imagesIEEE Transactions on Image Processing, 1997
- Direct recording of holograms by a CCD target and numerical reconstructionApplied Optics, 1994
- Iterative algorithms for twin-image elimination in in-line holography using finite-support constraintsJournal of the Optical Society of America A, 1993
- Reconstruction of an object from the modulus of its Fourier transformOptics Letters, 1978