Human CD8+CD25+ thymocytes share phenotypic and functional features with CD4+CD25+ regulatory thymocytes
- 1 December 2003
- journal article
- Published by American Society of Hematology in Blood
- Vol. 102 (12), 4107-4114
- https://doi.org/10.1182/blood-2003-04-1320
Abstract
CD8+CD25+ cells, which expressed high levels of Foxp3, glucocorticoid-induced tumor necrosis factor receptor (GITR), CCR8, tumor necrosis factor receptor 2 (TNFR2), and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) mRNAs, were identified in the fibrous septa and medullary areas of human thymus. Activated CD8+CD25+ thymocytes did not produce cytokines, but most of them expressed surface CTLA-4 and transforming growth factor β1 (TGF-β1). Like CD4+CD25+, CD8+CD25+ thymocytes suppressed the proliferation of autologous CD25-T cells via a contact-dependent mechanism. The suppressive activity of CD8+CD25+ thymocytes was abrogated by a mixture of anti-CTLA-4 and anti-TGF-β1 antibodies and it was mediated by their ability to inhibit the expression of the interleukin 2 receptor α chain on target T cells. These results demonstrate the existence of a subset of human CD8+CD25+ thymocytes sharing phenotype, functional features, and mechanism of action with CD4+CD25+ T regulatory cells. (Blood. 2003;102:4107-4114)Keywords
This publication has 39 references indexed in Scilit:
- Control of Regulatory T Cell Development by the Transcription Factor Foxp3Science, 2003
- Phenotype, Localization, and Mechanism of Suppression of CD4+CD25+ Human ThymocytesThe Journal of Experimental Medicine, 2002
- CD4+CD25+ Regulatory T Cells Can Mediate Suppressor Function in the Absence of Transforming Growth Factor β1 Production and ResponsivenessThe Journal of Experimental Medicine, 2002
- Generation of Human CD8 T Regulatory Cells by CD40 Ligand–activated Plasmacytoid Dendritic CellsThe Journal of Experimental Medicine, 2002
- CD4+CD25+ Immunoregulatory T CellsImmunity, 2002
- Stimulation of CD25+CD4+ regulatory T cells through GITR breaks immunological self-toleranceNature Immunology, 2002
- Cell Contact–Dependent Immunosuppression by Cd4+Cd25+Regulatory T Cells Is Mediated by Cell Surface–Bound Transforming Growth Factor βThe Journal of Experimental Medicine, 2001
- Major Histocompatibility Complex Class II–Positive Cortical Epithelium Mediates the Selection of Cd4+25+ Immunoregulatory T CellsThe Journal of Experimental Medicine, 2001
- Regulatory T Cells in AutoimmmunityAnnual Review of Immunology, 2000
- Regulatory T Cell Clones Induced by Oral Tolerance: Suppression of Autoimmune EncephalomyelitisScience, 1994