Evaluation of Distant Organ Effect of Renal Ischemia and Reperfusion with Claudin-5
- 1 January 2020
- journal article
- research article
- Published by Kare Publishing in Journal of Critical and Intensive Care
- Vol. 11 (1), 3-7
- https://doi.org/10.37678/dcybd.2020.2155
Abstract
Purpose: Claudins are the barrier in the cells. They can regulate intracellular permeability, form pores or increase water permeability. They have important effects in determining the permeability of epithelial cells. Mammals have 27 claudins grouped in eight subgroups. The distribution patterns are tissue-specific. They are located in contact areas between the epithelium. The aim of this study was to determine the results of claudin-5 level of experimental ischemia reperfusion injury in rats kidney and indirect effects of liver, lung and heart. Materials and Methods: Rats (average 250-300 grams) were divided into two equal groups, 7 rats in each group. The ischemia / reperfusion (IR) group (= experimental group) was administered ischemia to the kidney vessels of rats for 60 min. Sham group did not have ischemia. The rats in both groups were sacrified 24 hours after the occlusion of the rats. The liver, heart and lungs were removed and placed in containers containing 10% formalin. Slayts were evaluated with light microscope. Cytoplasmic membrane staining was considered positive. Four grades were evaluated. Results: Statistically, when sham and experimental group were compared in terms of staining intensity, moderate staining was found to be significantly lower in the experimental group (P = 0.031). It was noted that the stain of the sham group in the lungs and heart was greater than experimental group. Conclusion: In experimental renal IR injury, claudin-5 level in the lung and heart were more affected than in the liver.This publication has 20 references indexed in Scilit:
- Sustaining Cardiac Claudin-5 Levels Prevents Functional Hallmarks of Cardiomyopathy in a Muscular Dystrophy Mouse ModelMolecular Therapy, 2012
- Claudins in lung diseasesRespiratory Research, 2011
- Liver Injury Following Renal Ischemia Reperfusion in RatsTransplantation Proceedings, 2010
- Structure and Function of Extracellular Claudin DomainsAnnals of the New York Academy of Sciences, 2009
- The claudinsGenome Biology, 2009
- Ischemic acute kidney injury induces a distant organ functional and genomic response distinguishable from bilateral nephrectomyAmerican Journal of Physiology-Renal Physiology, 2007
- Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn's diseaseGut, 2007
- Tight Junctions and Cell PolarityAnnual Review of Cell and Developmental Biology, 2006
- Effects of transdifferentiation and EGF on claudin isoform expression in alveolar epithelial cellsJournal of Applied Physiology, 2005
- Size-selective loosening of the blood-brain barrier in claudin-5–deficient miceThe Journal of cell biology, 2003