Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA
- 29 August 2006
- journal article
- editorial material
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (19), 7054-7057
- https://doi.org/10.1021/ac060926a
Abstract
The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.Keywords
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