The modulatory effects of lipopolysaccharide‐stimulated B cells on differential T‐cell polarization
Open Access
- 5 September 2008
- journal article
- Published by Wiley in Immunology
- Vol. 125 (2), 218-228
- https://doi.org/10.1111/j.1365-2567.2008.02832.x
Abstract
Lipopolysaccharide (LPS) is a major component of environmental microbial products. Studies have defined the LPS dose as a critical determining factor in driving differential T-cell polarization but the direct effects of LPS on individual antigen-presenting cells is unknown. Here, we investigated the effects of LPS doses on naive B cells and the subsequent modulatory effects of these LPS-activated B cells on T-cell polarization. The LPS was able to induce a proliferative response starting at a dose of 100 ng/ml and was capable of enhancing antigen internalization at a dose of 1 μg/ml in naive B cells. Following LPS stimulation, up-regulation of the surface markers CD40, CD86, I-Ad, immunoglobulin M, CD54 and interleukin-10 production, accompanied by down-regulation of CD5 and CD184 (CXCR4) were observed in a LPS dose-dependent manner. Low doses (< 10 ng/ml) of LPS-activated B cells drove T helper type 2 polarization whereas high doses (> 0·1 μg/ml) of LPS-activated B cells resulted in T regulatory type 1 cell polarization. In conclusion, LPS-activated B cells acquire differential modulatory effects on T-cell polarization. Such modulatory effects of B cells are dependent on the stimulation with LPS in a dose-dependent manner. These observations may provide one of the mechanistic explanations for the influence of environmental microbes on the development of allergic diseases.Keywords
This publication has 76 references indexed in Scilit:
- TH1 cells control themselves by producing interleukin-10Nature Reviews Immunology, 2007
- CD4+CD25−Foxp3− Th1 cells are the source of IL-10–mediated immune suppression in chronic cutaneous leishmaniasisThe Journal of Experimental Medicine, 2007
- Selective suppression of Toll‐like receptor 4 activation by chemokine receptor 4FEBS Letters, 2004
- Dose‐dependent effects of endotoxins on allergen sensitization and challenge in the mouseClinical & Experimental Allergy, 2004
- Lipopolysaccharide-enhanced, Toll-like Receptor 4–dependent T Helper Cell Type 2 Responses to Inhaled AntigenThe Journal of Experimental Medicine, 2002
- Pathogen-specific T Regulatory 1 Cells Induced in the Respiratory Tract by a Bacterial Molecule that Stimulates Interleukin 10 Production by Dendritic CellsThe Journal of Experimental Medicine, 2002
- Normal B cells fail to secrete interleukin‐12European Journal of Immunology, 1997
- INDUCTION OF TH1 AND TH2 CD4+T CELL RESPONSES:The Alternative ApproachesAnnual Review of Immunology, 1997
- The role of indoor allergens in asthmaAllergy, 1995
- Hay fever, hygiene, and household size.BMJ, 1989