Self-Assembled Gold Nanoparticle Molecular Probes for Detecting Proteolytic Activity In Vivo
- 10 February 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Nano
- Vol. 4 (3), 1511-1520
- https://doi.org/10.1021/nn9017334
Abstract
Target-activatable fluorogenic probes based on gold nanoparticles (AuNPs) functionalized with self-assembled heterogeneous monolayers of dye-labeled peptides and poly(ethylene glycol) have been developed to visualize proteolytic activity in vivo. A one-step synthesis strategy that allows simple generation of surface-defined AuNP probe libraries is presented as a means of tailoring and evaluating probe characteristics for maximal fluorescence enhancement after protease activation. Optimal AuNP probes targeted to trypsin and urokinase-type plasminogen activator required the incorporation of a dark quencher to achieve 5- to 8-fold signal amplification. These probes exhibited extended circulation time in vivo and high image contrast in a mouse tumor model.Keywords
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