Surface modification of titanium‐based alloys with bioactive molecules using electrochemically fixed nucleic acids
Open Access
- 5 May 2006
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part B: Applied Biomaterials
- Vol. 80B (1), 146-155
- https://doi.org/10.1002/jbm.b.30579
Abstract
A new method of surface modification for titanium (alloys) with bioactive molecules was developed with the intention of providing a new basis of implant adaptation for particular requirements of certain medical indications. Nucleic acid single strands are fixed electrochemically via their termini (regiospecifically) by growing an oxide layer on Ti6Al7Nb anodically. It could be shown that they are accessible to subsequent hybridization with complementary strands at physiological pH. Amount of nucleic acids immobilized and hybridized were determined radioanalytically using 32P-labelled nucleic acids. Stable fixation was attained at and above potentials of 4 VSCE. Up to 4 pmol/cm2 of nucleic acid single strands could be immobilized and hybridization efficiencies up to 1.0 were reached. Hybridization efficiency was found to depend on surface density of immobilized oligonucleotides, while hybridization rates increased when MgCl2 was added. A conjugate consisting of an oligonucleotide complementary to the immobilized strand and the hexapeptide GRGDSP with RGD as an integrin recognition site was synthesized. This conjugate was able to bind to integrins on osteoblasts. It was shown that this conjugate binds to the anchor strand fixed on Ti6Al7Nb to an extent comparable with the unconjugated complementary strand. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007Keywords
This publication has 35 references indexed in Scilit:
- Comparison of metal release from various metallic biomaterials in vitroBiomaterials, 2005
- RGD‐containing peptide GCRGYGRGDSPG reduces enhancement of osteoblast differentiation by poly(L‐lysine)‐graft‐poly(ethylene glycol)‐coated titanium surfacesJournal of Biomedical Materials Research Part A, 2003
- Immobilization of RGD to 〈111〉 silicon surfaces for enhanced cell adhesion and proliferationBiomaterials, 2002
- Characterization of oxide layers on Ti6Al4V and titanium by streaming potential and streaming current measurementsColloids and Surfaces B: Biointerfaces, 2002
- Surface Structure and Coverage of an Oligonucleotide Probe Tethered onto a Gold Substrate and Its Hybridization Efficiency for a Polynucleotide TargetLangmuir, 2001
- A 5-year comparison of hydroxyapatite-coated titanium plasma–sprayed and titanium plasma–sprayed cylinder dental implantsOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 1999
- Thermodynamics of Duplex Formation and Mismatch Discrimination on Photolithographically Synthesized Oligonucleotide ArraysPublished by American Chemical Society (ACS) ,1997
- The effect of film formation conditions on the structure and composition of anodic oxide films on titaniumCorrosion Science, 1995
- Molecular Recognition of Amino Acids by RNA-Aptamers: An L-Citrulline Binding RNA Motif and Its Evolution into an L-Arginine BinderJournal of the American Chemical Society, 1994
- The anodic oxidation of valve metals—I. Determination of ionic transport numbers by α-spectrometryElectrochimica Acta, 1986